T7 RNA Polymerase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 SER-43   TYR-44  12.6 11.3 -36.0 62.9 13.0 6.5 -19.3
 TYR-44   GLU-45  14.0 13.0 -10.8 90.3 75.8 62.7 -14.2
 GLU-45   MET-46  14.9 12.5 169.7 24.3 35.0 95.5 -39.3
 MET-46   GLY-47  14.8 8.8 -154.4 -3.6 96.5 45.8 132.5
 GLY-47   GLU-48  18.2 8.4 149.5 13.3 97.9 153.9 -75.0
 GLU-48   ALA-49  20.4 9.6 -7.7 8.0 150.9 87.3 -2.1
 ALA-49   ARG-50  21.1 8.4 -9.6 13.8 111.4 76.1 -2.2
 ARG-50   PHE-51  17.5 4.7 13.0 -6.5 120.3 159.9 -0.9
 PHE-51   ARG-52  17.1 5.4 -9.2 20.7 95.2 52.4 -1.8
 ARG-52   LYS-53  20.6 6.9 -28.8 21.9 154.7 89.4 -1.2
 LYS-53   MET-54  20.4 5.7 -21.3 5.6 98.4 54.1 5.5
 MET-54   PHE-55  18.0 2.3 -55.8 44.2 70.7 14.2 10.5
 PRO-72   LEU-73  12.8 12.8 -145.5 -41.6 7.9 16.2 142.3
 LEU-73   ILE-74  11.3 11.2 -20.6 27.6 57.3 57.8 -4.4
 ILE-74   THR-75  11.0 9.6 -44.3 29.1 73.6 71.6 2.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 THR-256   ARG-257  14.1 14.3 -35.6 44.8 72.0 55.9 -8.0
 ARG-257   ALA-258  13.3 12.6 172.2 30.0 127.1 145.5 118.2
 ALA-258   GLY-259  11.7 10.3 -179.1 -124.0 113.3 41.6 -6.4
 GLY-259   ALA-260  9.6 13.3 -68.2 -1.7 172.9 66.0 -37.1
 ALA-260   LEU-261  9.7 14.5 -39.9 51.5 114.2 47.3 -5.9
 LEU-261   ALA-262  6.7 11.7 -58.8 -15.3 65.3 34.7 50.6
 ALA-262   GLY-263  9.0 10.6 21.5 0.1 71.9 148.2 -4.4
 GLY-263   ILE-264  10.1 13.5 17.0 -21.5 49.8 103.1 1.5
 ILE-264   SER-265  7.0 13.6 -63.2 -58.3 56.3 62.1 88.8
 SER-265   PRO-266  6.5 10.6 -103.2 -9.3 122.0 40.6 -173.5
 PRO-266   MET-267  6.1 10.6 -17.3 -17.1 111.6 46.1 -10.2
 MET-267   PHE-268  9.2 7.9 115.4 60.2 131.7 148.5 113.7
 PHE-268   GLN-269  11.2 10.5 12.6 0.2 154.2 149.2 -8.9

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees