Huc25 Fab Fragment Heavy Chain

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-120   THR-121  5.3 5.6 -13.1 22.4 125.3 120.0 52.7  THR-121   LYS-122  2.5 2.6 12.7 -26.1 95.8 99.6 27.5  LYS-122   GLY-123  5.6 5.5 -2.7 10.9 125.0 131.4 21.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees