Calpain-1 Catalytic Subunit

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PRO-63   VAL-64  8.4 8.0 -3.5 17.1 96.5 97.6 -15.9
 VAL-64   SER-65  9.5 9.2 9.0 6.7 135.6 124.4 -119.6
 SER-65   HIS-66  9.2 9.7 -8.9 -0.4 11.9 9.5 63.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LYS-84   TRP-85  4.3 4.2 -3.2 -2.4 107.3 113.8 -12.2
 TRP-85   LYS-86  3.8 3.4 -8.1 8.9 37.8 41.5 2.6
 LYS-86   ARG-87  3.9 3.5 5.2 -5.1 70.4 69.9 -4.2

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LYS-197   VAL-198  7.2 6.8 5.6 4.0 162.7 160.9 -70.1
 VAL-198   ASN-199  8.2 7.6 -8.7 -1.5 56.7 57.6 73.2
 ASN-199   GLY-200  4.9 4.6 -0.2 -2.7 109.3 109.8 23.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLU-203   ALA-204  4.4 4.7 4.8 -7.9 14.7 15.9 -10.5
 ALA-204   LEU-205  2.9 3.0 6.3 -4.7 74.9 76.0 7.1
 LEU-205   SER-206  2.2 2.6 -2.8 -5.6 94.1 96.0 10.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees