Gp32 Single Stranded DNA Binding Protein

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-145   LYS-146  8.1 7.8 -18.6 17.1 121.2 124.6 6.0  LYS-146   ILE-147  5.7 5.5 5.9 -11.7 18.0 15.4 -30.3  ILE-147   ASN-148  4.9 4.7 6.0 -7.8 62.6 61.0 -11.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  THR-165   CYS-166  3.2 3.1 -9.0 5.1 17.7 17.6 12.5  CYS-166   PRO-167  2.3 2.3 -2.9 2.7 87.9 86.9 -14.9  PRO-167   TRP-168  4.9 4.9 11.9 -23.3 119.8 121.1 40.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-178   GLN-179  6.4 6.8 3.6 -14.9 134.3 129.8 48.2  GLN-179   VAL-180  4.6 4.8 8.8 -4.1 110.9 117.0 -47.7  VAL-180   SER-181  4.1 4.4 1.0 7.0 129.7 124.0 -45.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-190   PHE-191  5.9 6.6 -9.0 0.6 28.6 26.7 54.1  PHE-191   LEU-192  5.5 6.1 -3.0 4.2 69.9 63.7 -15.6  LEU-192   ASN-193  8.5 8.9 0.2 -0.8 155.1 153.4 -13.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees