Ribonuclease HII

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-66   GLU-67  11.2 11.8 0.5 1.8 115.4 115.4 -6.4  GLU-67   LEU-68  8.5 9.0 1.9 3.5 83.6 78.9 10.0  LEU-68   PRO-69  5.9 6.5 -3.5 7.6 54.4 55.1 -9.3  PRO-69   PRO-70  4.1 5.1 -9.6 -0.5 114.9 120.9 -27.7  PRO-70   ASP-71  3.2 3.5 -10.3 16.9 100.1 107.3 -12.9  ASP-71   VAL-72  6.9 7.4 23.0 -42.8 25.6 22.9 -115.3  VAL-72   ILE-73  7.2 7.5 43.3 -26.5 40.4 46.5 66.6  ILE-73   GLY-74  4.4 4.2 155.4 -148.8 79.0 62.9 35.4  GLY-74   SER-75  7.2 5.7 45.2 11.0 67.0 45.9 213.2  SER-75   ARG-76  9.9 7.3 57.9 -111.9 23.2 20.4 -316.7  ARG-76   GLU-77  8.4 6.3 33.7 -21.7 47.2 74.9 28.3  GLU-77   GLY-78  10.5 7.7 113.5 -177.0 40.8 87.8 -139.4  GLY-78   THR-79  7.7 7.4 86.2 13.8 105.7 90.1 -173.6  THR-79   LEU-80  5.3 4.3 -42.7 -11.8 56.1 56.8 201.8  LEU-80   ASN-81  2.3 1.5 68.8 -19.8 53.5 59.9 158.6  ASN-81   GLU-82  0.2 1.0 -0.7 5.0 99.6 81.4 2.0  GLU-82   PHE-83  3.6 4.3 -15.5 7.5 34.9 29.1 35.5  PHE-83   GLU-84  4.2 4.3 -0.8 -11.8 85.5 87.3 -12.5  GLU-84   VAL-85  4.1 4.8 13.0 -9.0 52.0 51.8 24.7  VAL-85   GLU-86  6.1 6.9 6.9 -7.2 109.7 114.9 13.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-156   GLU-157  6.7 6.6 -11.8 7.0 19.7 17.6 28.9  GLU-157   LYS-158  8.9 8.6 3.9 -1.9 128.8 129.2 -11.0  LYS-158   LEU-159  6.6 5.9 -4.6 3.8 80.3 88.3 6.1  LEU-159   LYS-160  3.6 3.3 3.7 -8.9 132.8 124.2 23.4  LYS-160   GLU-161  6.3 6.2 5.5 -7.1 161.0 167.6 7.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees