DNA Polymerase I

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASN-700   MET-701  15.2 15.3 -4.3 0.1 44.6 41.9 39.1  MET-701   ARG-702  12.3 12.5 1.2 -1.2 113.4 109.9 7.3  ARG-702   ARG-703  10.4 10.7 4.2 -2.5 127.7 123.3 -1.9  ARG-703   GLN-704  13.9 14.2 3.6 -6.7 154.2 160.0 53.3  GLN-704   ALA-705  13.8 14.2 5.1 -1.7 123.2 123.4 -60.5  ALA-705   LYS-706  10.1 10.5 -5.1 6.0 67.5 74.5 -3.1  LYS-706   ALA-707  11.4 12.0 5.4 -7.3 146.0 142.3 10.9  ALA-707   VAL-708  14.0 14.6 1.0 0.1 141.4 147.8 -10.9  VAL-708   ASN-709  11.4 12.0 -0.2 -2.9 67.7 72.6 -11.1  ASN-709   PHE-710  9.5 10.5 4.6 5.1 127.1 114.6 -60.3  PHE-710   GLY-711  12.8 14.2 -6.1 1.1 30.6 17.3 49.0  GLY-711   ILE-712  12.8 14.0 8.9 -12.6 129.4 129.4 22.5  ILE-712   VAL-713  9.3 10.9 6.2 0.8 117.1 101.5 -25.6  VAL-713   TYR-714  10.7 13.0 -1.8 -25.8 48.2 48.6 194.8  TYR-714   GLY-715  13.7 15.8 16.7 -43.4 146.4 144.2 184.5  GLY-715   ILE-716  11.8 14.2 -66.2 6.7 94.2 83.5 7.1  ILE-716   SER-717  11.6 16.5 72.6 -0.4 158.9 107.3 -479.0  SER-717   ASP-718  13.1 16.8 22.1 -19.2 90.2 41.7 50.3  TYR-719   GLY-720  18.8 20.9 -79.7 76.6 133.3 130.8 -19.9  GLY-720   LEU-721  19.9 20.4 91.4 -123.7 32.9 23.6 -245.5  LEU-721   ALA-722  18.7 18.1 -173.1 159.1 113.3 119.0 -50.7  ALA-722   GLN-723  20.9 20.7 24.1 -66.5 49.2 65.4 -116.8  GLN-723   ASN-724  23.8 23.4 8.4 -1.0 4.8 36.4 63.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-747   ARG-748  2.9 3.0 2.0 -4.8 26.3 27.3 -19.8  ARG-748   TYR-749  1.7 1.8 5.1 -4.4 68.2 65.3 12.2  TYR-749   MET-750  2.2 2.0 0.1 0.6 66.9 64.9 21.4  MET-750   GLU-751  4.3 4.1 -0.1 3.8 155.3 152.2 24.6  GLU-751   ASN-752  3.5 3.4 -0.8 -1.9 132.6 135.2 -25.7  ASN-752   ILE-753  1.5 1.2 4.8 -7.2 74.2 72.2 -7.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees