Dna-Directed RNA Polymerase II 140Kd Polypeptide

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-1149   ARG-1150  1.0 0.2 -13.7 4.4 27.0 35.2 64.4  ARG-1150   LEU-1151  2.8 2.5 33.1 -38.2 90.4 88.0 -45.5  LEU-1151   MET-1152  6.4 5.7 0.0 27.1 113.8 118.8 57.7  MET-1152   GLU-1153  6.6 5.5 -41.1 36.4 48.9 49.3 37.8  GLU-1153   ALA-1154  8.8 8.5 51.0 -13.6 61.0 79.3 114.0  ALA-1154   SER-1155  8.8 10.0 -113.6 89.9 104.0 140.1 -74.6  SER-1155   ASP-1156  11.9 12.2 87.3 -93.2 34.7 42.6 25.9  ASP-1156   ALA-1157  11.7 12.5 21.4 -15.0 28.3 38.0 16.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees