T7 RNA Polymerase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLN-568   ASP-569  4.4 4.7 -1.6 -21.0 26.0 31.6 108.4
 ASP-569   ILE-570  5.1 5.6 4.6 3.8 132.8 131.9 -38.9
 ILE-570   TYR-571  2.7 3.1 3.8 -13.2 129.3 130.0 40.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-680   ILE-681  9.3 9.3 1.4 3.1 112.7 112.6 31.0
 ILE-681   TRP-682  5.8 5.7 -7.4 5.6 99.5 100.3 -19.2
 TRP-682   GLU-683  5.3 5.0 -11.0 -4.6 50.4 51.2 62.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees