4M5.3 Anti-Fluorescein Single Chain Antibody Fragment

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PRO-118   THR-119  0.2 0.9 3.0 1.7 70.6 64.3 32.5
 THR-119   VAL-120  3.9 3.1 12.9 -4.7 60.1 58.4 42.9
 VAL-120   SER-121  5.3 4.7 -2.1 0.2 104.5 103.6 9.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PHE-140   LEU-141  9.1 9.0 -11.0 20.1 88.9 99.3 24.0
 LEU-141   ASN-142  5.7 5.7 37.7 -29.9 13.2 6.0 67.8
 ASN-142   ASN-143  5.0 4.7 -18.3 15.7 109.6 119.1 -18.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ASP-148   ILE-149  11.0 12.0 8.0 5.9 49.3 51.6 73.9
 ILE-149   ASN-150  9.0 9.7 -12.8 35.5 128.8 142.9 149.5
 ASN-150   VAL-151  9.7 10.1 20.5 -34.5 58.2 65.3 -39.9
 VAL-151   LYS-152  8.4 8.9 -35.8 37.1 157.3 158.7 -0.2

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-165   ASN-166  17.9 17.8 6.1 0.3 58.5 56.7 51.8
 ASN-166   SER-167  15.2 15.2 -14.9 -1.4 162.4 161.7 -118.1
 SER-167   TRP-168  15.1 15.4 2.3 8.2 62.0 55.5 70.1
 TRP-168   THR-169  12.7 13.0 -5.7 25.9 163.7 151.9 151.3
 THR-169   ASP-170  11.7 12.2 11.1 -11.7 60.8 51.3 -49.1
 ASP-170   GLN-171  11.6 12.2 -4.1 -2.5 95.4 98.8 4.4

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 SER-176   THR-177  4.8 5.0 4.9 3.4 7.7 6.9 64.4
 THR-177   TYR-178  4.2 4.5 -11.3 11.2 104.8 107.5 -5.6
 TYR-178   SER-179  6.3 6.8 -3.4 3.6 130.4 134.0 15.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 CYS-199   GLU-200  6.9 6.8 25.4 5.3 83.7 78.2 65.1
 GLU-200   ALA-201  4.0 4.3 -21.1 36.4 124.1 141.1 81.3
 ALA-201   THR-202  4.8 4.6 -16.0 -14.9 94.8 92.1 11.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 SER-208   PRO-209  6.9 7.1 0.0 -15.2 62.5 62.3 24.5
 PRO-209   ILE-210  3.9 3.9 25.5 -29.2 103.0 100.7 39.0
 ILE-210   VAL-211  1.1 0.9 2.6 -1.9 57.1 55.6 20.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees