Isocitrate Dehydrogenase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-124   TYR-125  2.1 2.1 -4.8 9.0 78.0 72.6 5.5  TYR-125   ILE-126  3.6 3.8 1.5 3.9 150.9 152.8 -12.5  ILE-126   CYS-127  3.6 3.5 -1.0 -6.0 38.2 42.8 20.2  CYS-127   LEU-128  3.8 3.8 -18.1 15.4 46.3 40.7 14.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ARG-132   TYR-133  5.3 5.1 2.9 2.4 34.4 32.8 15.9  TYR-133   TYR-134  4.5 4.3 -1.4 1.1 114.6 109.9 17.5  TYR-134   GLN-135  2.6 2.3 1.3 1.0 12.5 7.0 8.0  GLN-135   GLY-136  3.4 3.3 3.6 -15.0 64.4 62.8 -27.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-319   ILE-320  5.2 8.8 150.3 -8.3 138.1 143.0 -672.5  ILE-320   GLY-321  8.3 8.9 41.9 -4.0 100.0 81.5 -25.2  GLY-321   ILE-322  8.9 8.6 1.1 -6.3 106.2 93.1 -15.8  ILE-322   ALA-323  5.8 4.8 7.4 -43.7 132.2 137.0 123.5  ALA-323   PRO-324  4.4 4.4 -22.5 0.0 40.5 33.4 85.3  PRO-324   GLY-325  2.9 3.1 6.5 -0.3 147.3 146.7 -22.8  GLY-325   ALA-326  2.5 2.4 12.1 0.2 141.8 138.5 -45.3  ALA-326   ASN-327  2.2 2.3 -4.1 8.1 35.2 33.1 -11.4  ASN-327   ILE-328  2.1 2.6 -6.1 4.9 55.7 55.0 -3.4  ILE-328   GLY-329  1.8 1.7 -6.6 0.4 22.9 22.4 34.5  GLY-329   ASP-330  2.1 2.0 -11.5 2.2 70.3 71.3 7.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees