Histidyl-Trna Synthetase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-169   GLU-170  11.1 11.2 -7.2 8.3 27.4 16.4 3.9  GLU-170   ASP-171  11.4 10.8 -19.7 11.9 57.3 65.9 39.4  ASP-171   ARG-172  7.7 7.0 -2.5 9.5 71.0 82.0 -19.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-227   PHE-228  1.7 2.5 -6.5 0.0 92.8 99.6 -14.5  PHE-228   LEU-229  2.2 1.4 -3.1 -2.2 30.2 38.3 29.0  LEU-229   GLY-230  3.1 2.7 11.0 -4.8 83.3 76.8 26.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-255   ALA-256  8.3 8.7 -20.8 -79.2 81.0 97.7 49.0  ALA-256   LEU-257  11.2 8.8 136.3 -40.8 43.1 45.3 424.7  LEU-257   VAL-258  10.4 10.3 -32.2 54.8 75.5 90.3 -7.6  VAL-258   ARG-259  10.1 12.0 -60.3 62.9 126.4 139.1 29.6  ARG-259   GLY-260  11.3 13.4 39.9 7.9 127.5 105.5 -128.7  GLY-260   LEU-261  13.5 16.9 37.4 -59.3 69.8 75.6 -58.4  LEU-261   ASP-262  15.1 16.4 -0.1 -24.8 94.0 60.9 11.4  ASP-262   TYR-263  14.6 15.6 21.8 3.3 30.2 57.6 87.5  TYR-263   TYR-264  13.9 14.6 -4.0 17.8 107.4 97.6 -20.7  TYR-264   VAL-265  10.7 11.0 8.3 -9.9 111.9 122.2 -41.0  VAL-265   ARG-266  7.6 8.4 -20.6 27.4 108.1 110.0 -23.6  ARG-266   THR-267  3.9 4.6 6.9 -15.1 74.3 72.2 -32.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees