T7 RNA Polymerase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-73   ILE-74  5.5 5.1 -55.5 74.7 82.9 81.9 -203.5
 ILE-74   THR-75  8.3 8.4 -44.8 25.1 97.9 78.8 196.1
 THR-75   THR-76  10.7 9.4 -26.3 0.2 173.6 146.9 -13.9
 THR-76   LEU-77  10.0 9.8 42.5 -19.1 67.6 51.7 11.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ALA-255   THR-256  8.3 7.7 20.0 -18.5 75.5 78.5 3.3
 THR-256   ARG-257  7.6 7.0 16.7 28.9 44.1 32.3 25.8
 ARG-257   ALA-258  5.2 4.5 127.2 17.7 22.3 38.0 82.7
 ALA-258   GLY-259  3.9 6.4 -146.8 -92.0 102.9 97.2 13.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees