Taq DNA Polymerase
(All numbering and residues are taken from first PDB file)
Bending Residue Dihedral Analysis
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
HIS-283
GLU-284
17.9
19.4
-85.2
54.1
81.9
112.9
-2.2
GLU-284
PHE-285
17.2
19.8
-41.4
17.2
150.6
159.4
-15.1
PHE-285
GLY-286
16.2
22.0
-4.4
1.1
95.1
136.1
3.0
GLY-286
LEU-287
12.6
19.2
169.4
29.5
101.2
67.7
84.7
LEU-287
LEU-288
9.6
17.5
-137.7
-3.8
37.1
131.8
7.1
LEU-288
GLU-289
7.5
19.7
-174.4
43.1
99.4
153.6
-91.8
GLU-289
SER-290
3.9
18.8
-96.5
74.1
24.9
92.1
2.8
SER-290
PRO-291
3.4
15.1
8.5
57.0
92.6
31.5
25.6
PRO-291
LYS-292
4.8
12.8
-46.2
90.2
21.2
86.3
-2.5
LYS-292
ALA-293
6.6
12.7
115.9
36.9
97.6
124.9
55.5
ALA-293
LEU-294
7.6
11.8
25.6
36.2
100.0
64.0
5.9
LEU-294
GLU-295
6.4
9.1
19.3
-0.2
110.3
117.9
-0.1
GLU-295
GLU-296
9.0
10.5
-37.0
11.7
5.9
20.3
13.6
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees