Type IIe Restriction Endonuclease Naei

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-43   TYR-44  4.0 3.6 -13.5 2.0 26.0 29.9 89.4
 TYR-44   ASP-45  3.9 3.7 -1.3 -4.3 96.5 95.8 6.6
 ASP-45   GLY-46  5.8 5.7 12.7 -12.5 146.3 151.5 -1.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LYS-62   THR-63  11.5 11.8 -5.9 -18.8 25.0 40.9 167.7
 THR-63   HIS-64  13.2 14.2 11.7 -56.4 126.8 144.9 219.8
 HIS-64   MET-65  10.0 11.5 48.5 -8.6 87.4 81.5 24.4
 MET-65   GLY-66  8.1 8.7 -4.6 2.5 38.0 49.0 -2.5
 GLY-66   THR-67  10.1 9.9 16.1 -8.8 141.9 154.1 -51.7
 THR-67   LEU-68  8.4 8.8 19.0 -11.8 84.9 89.1 -31.0
 LEU-68   VAL-69  5.0 5.2 13.6 -11.0 98.4 93.7 -44.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 HIS-176   ILE-177  6.4 6.5 42.9 -51.4 150.4 140.8 46.2
 ILE-177   PRO-178  5.4 5.4 5.2 -9.3 127.9 128.3 10.1
 PRO-178   GLY-179  4.5 4.0 3.0 -1.9 84.7 80.1 -4.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees