Fatty Acid Synthase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLN-608   CYS-609  10.3 10.4 -2.5 3.8 110.8 111.3 11.7  CYS-609   ILE-610  7.0 7.1 -11.7 13.4 76.4 75.0 -1.6  ILE-610   LYS-611  5.8 5.6 -5.0 0.3 132.3 131.0 -33.6  LYS-611   GLU-612  8.6 8.3 -6.7 19.6 155.3 155.9 85.2  GLU-612   ALA-613  6.7 6.7 -32.9 34.6 95.0 99.3 82.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-641   CYS-642  3.5 3.6 -3.6 10.7 125.9 127.0 60.3  CYS-642   HIS-643  1.9 2.1 -5.6 10.4 162.1 163.2 39.0  HIS-643   ASN-644  1.4 1.5 1.3 1.1 58.5 56.7 6.4  ASN-644   SER-645  3.2 3.4 -2.1 -0.8 174.5 173.8 -15.4  SER-645   LYS-646  2.9 3.1 -0.4 1.7 106.6 103.6 -5.9  LYS-646   ASP-647  3.5 3.9 -1.3 -1.7 72.6 71.0 -11.6  ASP-647   THR-648  7.1 7.4 5.4 -4.8 146.1 142.3 19.4  THR-648   VAL-649  7.2 7.5 18.7 -11.1 139.1 141.9 -55.6  VAL-649   THR-650  6.1 6.4 0.3 5.5 144.8 144.0 -2.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PHE-686   MET-687  5.2 4.7 26.6 -18.7 116.1 106.2 -88.4  MET-687   GLU-688  1.6 1.3 0.3 -8.4 121.8 116.1 41.3  GLU-688   ALA-689  4.7 4.7 2.2 -2.0 164.2 165.3 8.0  ALA-689   ILE-690  3.6 3.6 1.4 -0.8 99.7 99.6 -0.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees