Poly(a) Polymerase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 GLU-32   LEU-33  3.9 3.7 1.5 -9.6 140.1 138.9 47.4
 LEU-33   LYS-34  5.6 5.4 12.9 -7.2 74.8 76.9 16.1
 LYS-34   LYS-35  8.8 8.7 4.8 -9.5 50.6 54.4 -38.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 ASP-195   GLU-196  8.1 8.3 -0.6 -2.2 96.1 98.3 65.7
 GLU-196   ILE-197  5.5 6.0 -0.4 2.9 29.9 31.4 0.7
 ILE-197   LEU-198  3.9 4.4 3.2 -9.4 123.2 123.8 -0.9

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 PRO-202   LYS-203  11.6 12.3 -15.7 12.4 128.9 126.6 20.6
 LYS-203   PRO-204  11.3 12.0 0.8 -7.4 81.0 81.4 -48.1
 PRO-204   ASN-205  9.5 10.0 0.3 16.4 20.1 23.3 207.2
 ASN-205   VAL-206  9.8 10.5 -10.3 -3.3 61.4 62.3 173.7
 ASN-205   VAL-206  9.8 10.5 -10.3 -3.3 61.4 62.3 173.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 ASN-205   VAL-206  9.8 10.5 -10.3 -3.3 61.4 62.3 173.7
 ASN-205   VAL-206  9.8 10.5 -10.3 -3.3 61.4 62.3 173.7
 VAL-206   PHE-207  8.0 8.6 4.0 -8.8 66.8 67.1 31.9
 PHE-207   ARG-208  5.3 5.9 9.9 -2.9 111.8 109.2 -22.6
 ARG-208   ILE-209  5.7 6.0 -4.4 5.3 154.2 150.1 34.4
 ARG-208   ILE-209  5.7 6.0 -4.4 5.3 154.2 150.1 34.4

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 ARG-208   ILE-209  5.7 6.0 -4.4 5.3 154.2 150.1 34.4
 ARG-208   ILE-209  5.7 6.0 -4.4 5.3 154.2 150.1 34.4
 ILE-209   ALA-210  4.7 5.0 0.9 -10.3 93.3 92.5 -0.7
 ALA-210   LEU-211  1.9 2.4 5.6 5.2 113.7 118.0 -36.4
 LEU-211   ARG-212  1.7 1.6 -12.1 7.4 126.6 129.7 15.7
 ARG-212   ALA-213  4.4 4.2 -0.9 -0.4 127.9 125.5 -10.2

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 PRO-276   ILE-277  8.8 9.2 -1.4 -7.4 59.6 59.8 -0.9
 ILE-277   GLU-278  6.5 6.6 -7.9 3.6 30.0 26.3 114.9
 GLU-278   ASP-279  4.7 5.5 19.4 -15.5 141.0 137.1 -129.6
 ASP-279   GLY-280  3.7 4.1 -7.7 8.8 79.2 75.8 83.2
 GLY-280   PRO-281  1.3 2.2 16.2 6.6 149.5 149.8 -180.4
 PRO-281   LEU-282  2.3 1.8 -136.1 155.3 77.8 103.3 112.1
 LEU-282   GLN-283  4.2 4.2 -38.3 38.9 72.2 80.1 36.8
 GLN-283   VAL-284  6.3 6.4 -31.3 19.3 137.6 145.2 -104.3
 VAL-284   ARG-285  7.8 6.9 -15.2 -10.7 110.6 93.2 -140.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees