Humanized Antibody D3h44

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-111   SER-112  6.0 5.6 0.9 4.0 81.3 74.3 8.2  SER-112   SER-113  4.7 3.3 3.2 10.0 20.0 14.0 24.8  SER-113   ALA-114  5.0 3.0 -18.5 -4.5 88.8 95.5 -7.0  ALA-114   SER-115  5.0 2.7 13.9 1.0 65.3 80.8 18.1  SER-115   THR-116  5.3 4.1 12.0 -13.0 132.4 139.1 2.1  THR-116   LYS-117  7.0 6.7 17.2 5.9 89.3 86.0 10.6  LYS-117   GLY-118  10.2 9.7 1.1 -5.8 148.7 140.1 7.0  GLY-118   PRO-119  12.9 12.7 -3.4 -5.3 60.2 56.2 13.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees