Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-85   GLY-86  7.4 6.8 -20.0 -1.9 70.5 70.5 4.3  GLY-86   PRO-87  3.7 3.2 30.7 -3.3 95.2 119.1 -53.0  PRO-87   GLU-88  3.9 3.5 -99.0 36.9 25.8 33.2 229.2  GLU-88   ILE-89  6.4 5.6 -2.8 -9.8 79.2 90.6 -68.5  ILE-89   ARG-90  8.8 8.8 18.8 -0.5 115.4 115.1 -36.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-177   ASN-178  8.9 9.2 -33.6 25.8 87.0 84.6 36.5  ASN-178   LEU-179  6.2 6.0 -26.9 12.3 111.4 112.4 -0.4  LEU-179   PRO-180  7.7 7.1 32.3 -25.5 66.8 85.2 -34.9  PRO-180   GLY-181  6.4 5.9 171.5 165.1 90.3 89.4 44.4  GLY-181   CYS-182  10.1 8.8 -39.3 65.2 22.5 48.7 -37.3  CYS-182   ASP-183  10.4 10.7 -131.1 115.3 39.5 18.9 60.7  ASP-183   VAL-184  10.6 10.0 -19.8 10.0 46.0 45.9 32.4  VAL-184   ASP-185  8.6 9.1 -17.0 45.7 22.8 23.4 -110.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-187   ALA-188  5.5 5.7 -11.5 1.2 103.4 108.1 -10.8  ALA-188   VAL-189  3.3 3.5 -18.6 27.3 78.6 68.3 40.6  VAL-189   SER-190  0.3 0.8 -36.3 18.9 72.5 68.1 20.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees