Crispr-Associated Endonuclease Cas9/csn1
(All numbering and residues are taken from first PDB file)
Bending Residue Dihedral Analysis
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
GLU-762
MET-763
11.1
10.2
-17.1
21.2
103.4
116.7
7.4
MET-763
ALA-764
8.2
7.7
8.3
-4.8
15.7
7.9
3.5
ALA-764
ARG-765
8.9
6.5
-161.4
-18.8
127.6
124.2
-131.4
ASN-776
SER-777
12.5
7.8
33.7
-36.6
12.7
99.4
4.5
SER-777
ARG-778
14.1
9.7
63.4
-17.5
68.6
47.5
-167.4
ARG-778
GLU-779
15.7
11.3
157.3
15.0
84.1
71.6
-5.5
GLU-779
ARG-780
12.8
12.2
-175.6
9.6
143.8
163.9
184.9
ARG-780
MET-781
10.7
10.7
7.9
-6.7
31.5
32.4
4.3
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
LEU-908
SER-909
19.8
19.7
-10.5
2.9
103.6
117.1
0.9
SER-909
GLU-910
23.1
22.6
16.6
-18.2
50.7
46.7
-3.3
GLU-910
LEU-911
24.6
23.2
29.6
-8.6
21.7
38.5
18.8
LEU-911
ASP-912
23.8
22.1
9.6
-14.9
87.2
83.0
1.4
ASP-912
LYS-913
20.1
18.4
103.4
-8.2
84.9
68.6
43.6
LYS-913
ALA-914
20.6
18.7
1.0
110.8
15.9
81.7
97.0
ALA-914
GLY-915
21.9
15.1
129.1
-37.1
56.6
62.0
-120.2
GLY-915
PHE-916
19.0
15.5
32.7
-9.2
95.4
70.7
-24.2
PHE-916
ILE-917
16.6
11.9
-165.1
-63.5
121.6
54.3
-34.7
ILE-917
LYS-918
18.8
10.2
173.8
35.6
10.2
68.6
149.4
LYS-918
ARG-919
18.6
6.5
-9.4
11.1
102.1
147.4
-2.1
ARG-919
GLN-920
14.8
7.6
-12.7
1.1
84.4
143.2
-9.6
GLN-920
LEU-921
15.0
8.0
-0.5
49.6
152.1
96.9
39.2
LEU-921
VAL-922
16.1
4.9
-44.0
37.6
140.5
114.3
-2.9
VAL-922
GLU-923
14.9
2.8
-148.4
8.0
84.1
158.1
-93.0
GLU-923
THR-924
11.2
5.0
-160.8
44.2
92.8
123.9
-64.9
THR-924
ARG-925
10.1
4.1
-29.9
-0.8
96.9
98.9
12.2
ARG-925
GLN-926
6.3
0.9
-170.9
1.5
148.8
135.2
140.7
GLN-926
ILE-927
6.0
2.3
-10.0
-2.3
135.2
155.8
-9.7
ILE-927
THR-928
2.6
2.3
4.1
-0.9
53.8
72.6
1.2
THR-928
LYS-929
4.7
2.4
-9.8
1.9
130.5
105.0
-6.8
LYS-929
HIS-930
6.9
3.4
0.4
-10.3
40.1
27.7
-6.7
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees