Bifunctional P-450/nadph-P450 Reductase
(All numbering and residues are taken from first PDB file)
Bending Residue Dihedral Analysis
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
ARG-167
ASP-168
11.6
11.6
-1.2
-8.9
13.3
13.5
77.2
ASP-168
GLN-169
11.4
11.0
18.4
-16.6
105.4
99.9
-54.0
GLN-169
PRO-170
7.7
7.4
-2.2
10.6
128.3
139.5
40.8
PRO-170
HIS-171
5.1
4.8
-1.3
2.4
150.4
152.6
-15.5
HIS-171
PRO-172
4.8
4.9
8.3
-9.1
85.8
84.5
8.7
PRO-172
PHE-173
3.1
3.9
1.4
5.9
69.3
69.6
2.1
PHE-173
ILE-174
0.5
0.6
2.6
-13.6
140.9
136.9
36.4
ILE-174
THR-175
2.4
1.4
8.4
-8.3
124.5
130.4
-8.9
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
ASP-214
LEU-215
3.9
4.0
-12.0
1.1
90.7
90.1
19.9
LEU-215
VAL-216
0.5
0.8
-0.7
-5.6
43.5
48.5
17.7
VAL-216
ASP-217
2.6
2.4
0.5
6.1
153.8
156.5
-9.3
ASP-217
LYS-218
1.5
1.3
-8.4
-1.1
81.3
79.1
16.9
LYS-218
ILE-219
2.5
2.7
-2.2
8.1
75.1
83.7
6.6
ILE-219
ILE-220
4.0
4.2
-5.7
4.1
14.6
19.4
18.5
ILE-220
ALA-221
3.1
3.8
6.3
-21.7
127.0
133.3
37.7
ALA-221
ASP-222
2.9
3.1
9.9
-3.0
94.7
90.9
-18.7
ASP-222
ARG-223
6.4
6.7
5.0
4.2
126.3
116.8
-33.8
ARG-223
LYS-224
7.4
7.9
-11.5
7.2
15.9
6.7
29.6
LYS-224
ALA-225
5.4
6.3
9.0
-14.7
108.5
116.4
15.1
ALA-225
SER-226
7.7
7.9
9.8
-6.4
94.4
89.8
-10.0
SER-230
ASP-231
15.9
16.0
13.2
-7.9
102.0
95.1
-12.0
ASP-231
ASP-232
17.5
17.7
1.3
-0.8
26.6
17.9
-8.1
ASP-232
LEU-233
16.3
16.4
-4.9
5.8
91.0
89.4
-9.3
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees