Igg2a Fab Fragment (50.1)

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-100   GLY-101  6.2 6.4 5.5 2.5 33.5 34.4 52.2  GLY-101   THR-102  3.5 3.8 -18.6 24.2 138.9 134.6 -33.5  THR-102   LYS-103  3.2 3.1 -14.7 13.5 129.0 136.3 -65.1  LYS-103   LEU-104  2.0 2.0 -1.6 22.3 131.2 132.5 57.3  LEU-104   GLU-105  1.6 1.4 24.7 -2.3 30.3 36.0 30.3  GLU-105   ILE-106  0.4 0.3 13.2 -12.7 49.4 50.6 -79.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees