Ribonuclease III

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-145   GLY-146  12.9 13.1 28.9 -35.5 66.0 70.9 -0.2  GLY-146   ARG-147  10.4 10.1 -139.0 30.2 162.4 158.2 -191.7  ARG-147   VAL-148  10.0 11.2 -32.7 -15.4 128.2 91.5 -21.8  VAL-148   LYS-149  8.7 9.4 91.5 -17.3 43.5 12.6 128.1  LYS-149   LYS-150  7.2 9.3 -14.6 33.8 159.4 108.8 27.9  LYS-150   ASP-151  6.8 6.4 -1.9 11.4 105.2 78.2 -1.3  ASP-151   TYR-152  3.5 3.6 97.6 -6.5 27.8 33.5 145.4  TYR-152   LYS-153  4.3 4.2 -5.9 -1.5 121.7 121.7 -0.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees