Crispr-Associated Endonuclease Cas9/csn1

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  THR-445   PHE-446  11.9 11.4 -18.5 -11.5 22.0 16.1 16.5  PHE-446   ARG-447  10.1 10.1 -6.2 2.6 51.4 63.2 -1.9  PRO-503   ASN-504  13.1 11.5 -20.0 125.7 128.2 83.4 -114.6  ASN-504   GLU-505  10.6 10.6 40.5 -49.3 31.8 69.7 -5.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees