Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-714   GLY-715  8.2 7.8 -11.8 -3.5 73.5 75.1 44.6  GLY-715   PRO-716  4.5 4.1 11.6 -15.4 90.8 103.3 20.1  PRO-716   GLU-717  2.1 2.1 -90.0 80.4 40.2 32.7 59.3  GLU-717   ILE-718  5.0 5.0 -1.1 11.6 89.1 90.6 21.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-816   LEU-817  8.5 7.9 9.2 -2.4 125.6 133.9 13.4  LEU-817   PRO-818  5.3 5.1 4.5 -4.2 121.5 119.8 3.6  PRO-818   ALA-819  3.8 3.2 -12.1 1.2 116.0 114.8 -15.3  ALA-819   VAL-820  4.1 3.0 7.7 -10.8 99.5 118.4 -0.7  VAL-820   SER-821  2.4 2.1 -25.8 -10.2 72.7 60.7 97.1  SER-821   GLU-822  2.5 2.3 -3.0 9.5 22.3 33.0 -23.0  GLU-822   LYS-823  4.3 4.4 -0.9 -1.4 95.6 103.7 -11.0  LYS-823   ASP-824  7.2 7.2 -4.7 -6.3 94.9 98.5 12.4  ASP-824   ILE-825  6.2 6.1 18.1 -10.4 149.2 148.8 -15.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees