Alcohol Dehydrogenase E Chain

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-179   TYR-180  4.4 4.8 -56.5 40.0 108.2 106.6 22.4  TYR-180   GLY-181  5.2 5.5 6.0 -10.6 24.2 33.3 -70.8  GLY-181   SER-182  3.6 3.9 19.8 -14.4 42.3 29.8 84.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-291   VAL-292  9.3 9.1 -7.3 36.6 132.2 136.8 321.1  VAL-292   GLY-293  8.1 7.6 -33.4 -39.9 118.5 107.7 -428.1  GLY-293   VAL-294  11.4 10.1 -9.2 -52.4 65.7 54.4 413.5  VAL-294   PRO-295  13.4 13.0 -11.1 49.8 87.0 111.3 82.9  PRO-295   PRO-296  17.0 16.2 -42.6 -17.8 98.8 51.7 149.1  PRO-296   ASP-297  20.3 17.4 -154.6 -43.7 43.5 71.5 -1375.1  ASP-297   SER-298  20.8 20.7 -51.2 142.9 36.1 54.6 2125.6  SER-298   GLN-299  21.8 20.7 145.8 1.0 112.7 160.9 -983.9  GLN-299   ASN-300  22.0 22.0 19.5 55.2 132.2 113.1 -516.8  ASN-300   LEU-301  20.8 19.3 135.3 -131.2 85.2 76.6 250.6  LEU-301   SER-302  20.2 19.8 -15.3 31.1 18.6 18.3 -167.5  SER-302   MET-303  21.6 21.7 -9.7 -11.1 82.6 68.0 54.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ARG-312   THR-313  12.4 12.2 -4.8 4.4 91.2 102.5 -13.3  THR-313   TRP-314  8.9 8.9 -2.3 12.2 144.4 144.4 112.6  TRP-314   LYS-315  8.8 8.6 -3.9 29.0 103.8 99.4 219.2  LYS-315   GLY-316  6.0 5.3 53.8 -79.7 25.2 53.0 -214.2  GLY-316   ALA-317  6.1 5.2 -27.5 1.4 108.1 107.7 -47.1  ALA-317   ILE-318  4.3 3.3 -17.9 6.6 151.5 153.9 -61.6  ILE-318   PHE-319  5.6 5.1 -9.9 3.7 120.1 119.4 83.2  PHE-319   GLY-320  3.8 3.7 51.6 -57.3 109.4 115.8 44.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees