Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-714   GLY-715  8.1 7.7 -11.1 -4.2 73.2 75.3 41.4  GLY-715   PRO-716  4.5 4.0 12.5 -14.0 91.6 104.1 19.0  PRO-716   GLU-717  2.1 2.1 -90.5 81.7 39.6 32.9 58.5  GLU-717   ILE-718  5.0 5.0 -0.6 6.8 88.3 88.8 20.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-816   LEU-817  8.5 7.9 0.2 7.6 125.7 134.3 19.8  LEU-817   PRO-818  5.4 5.2 3.7 -2.0 122.3 122.0 7.0  PRO-818   ALA-819  3.9 3.3 -13.3 1.0 115.3 114.6 -16.4  ALA-819   VAL-820  4.1 3.0 7.1 -11.2 99.6 117.0 -1.8  VAL-820   SER-821  2.3 2.0 -27.3 -9.0 73.4 61.6 102.7  SER-821   GLU-822  2.5 2.3 -0.4 8.7 22.0 32.8 -33.5  GLU-822   LYS-823  4.3 4.5 -2.8 0.0 95.1 103.3 -6.5  LYS-823   ASP-824  7.2 7.2 -4.9 -4.7 94.1 97.2 10.7  ASP-824   ILE-825  6.1 6.0 19.1 -14.0 150.0 149.3 -12.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees