Nickel-Binding Periplasmic Protein

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-344   SER-345  3.9 4.2 -4.2 -10.2 82.4 85.9 -20.4  SER-345   PHE-346  7.3 7.4 2.7 9.1 65.2 57.0 158.0  PHE-346   ILE-347  8.8 9.0 -0.8 7.8 69.1 72.6 -207.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-352   SER-353  13.4 13.5 -7.4 -5.3 83.2 84.7 52.5  SER-353   LYS-354  10.2 10.3 -1.5 11.3 154.6 153.5 101.8  LYS-354   SER-355  9.9 9.8 0.8 1.5 77.5 79.8 -184.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-373   ILE-374  4.5 5.0 3.6 7.8 93.6 89.8 7.3  ILE-374   GLY-375  8.1 8.5 -4.5 -3.2 119.8 117.0 -68.3  GLY-375   GLU-376  10.4 10.5 -17.9 11.6 88.4 88.4 50.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-391   MET-392  7.5 7.6 2.2 -1.6 125.5 123.6 4.7  MET-392   ILE-393  4.1 4.2 6.9 -2.9 90.2 87.1 -39.1  ILE-393   PHE-394  5.1 5.1 -3.3 -5.9 56.9 61.6 46.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees