Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-1916   GLU-1917  7.7 6.9 -11.3 46.3 126.9 121.9 68.6  GLU-1917   ILE-1918  5.3 5.1 -10.3 -13.8 57.2 61.3 26.9  ILE-1918   ARG-1919  3.1 2.6 -3.4 3.5 108.6 111.3 26.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-2008   ASN-2009  3.8 3.4 3.0 3.0 105.5 103.7 35.7  ASN-2009   LEU-2010  1.0 1.2 13.9 -2.8 90.0 85.4 -39.9  LEU-2010   PRO-2011  3.9 4.7 13.7 -14.5 118.0 121.1 -36.6  PRO-2011   GLY-2012  5.3 6.1 -155.2 -170.5 100.7 99.9 72.2  GLY-2012   ALA-2013  9.0 9.7 -36.2 27.2 165.2 154.0 19.1  ALA-2013   ALA-2014  8.4 9.4 -4.0 -3.7 124.9 103.6 -102.7  ALA-2014   VAL-2015  9.3 10.5 0.0 42.5 125.5 137.8 115.8  VAL-2015   ASP-2016  6.4 7.3 33.2 -16.3 66.5 47.0 73.8  VAL-2015   ASP-2016  6.4 7.3 33.2 -16.3 66.5 47.0 73.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-2015   ASP-2016  6.4 7.3 33.2 -16.3 66.5 47.0 73.8  VAL-2015   ASP-2016  6.4 7.3 33.2 -16.3 66.5 47.0 73.8  ASP-2016   LEU-2017  8.3 8.5 -72.5 46.3 121.5 120.9 -75.4  LEU-2017   PRO-2018  7.3 6.7 -6.2 12.3 89.5 87.7 3.0  PRO-2018   ALA-2019  10.5 9.9 -6.2 -1.6 45.1 38.0 28.2  ALA-2019   VAL-2020  12.9 12.1 -2.4 5.5 110.7 108.4 -3.0  VAL-2020   SER-2021  13.5 13.2 -11.0 10.0 145.4 149.6 -7.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees