Dna-Directed RNA Polymerase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 TYR-44   GLU-45  14.1 13.8 17.9 -97.4 69.6 76.3 -17.8
 GLU-45   MET-46  13.9 14.7 165.9 -1.5 91.4 145.5 -43.3
 MET-46   GLY-47  10.3 14.6 134.2 29.0 47.4 96.5 137.1
 GLY-47   GLU-48  10.0 18.0 -177.2 0.9 156.2 97.4 -81.8
 GLU-48   ALA-49  11.2 20.2 38.2 -31.5 85.8 151.4 -5.8
 ALA-49   ARG-50  9.6 20.9 3.1 -26.7 79.3 111.7 -0.6
 ARG-50   PHE-51  6.3 17.3 6.9 2.9 14.6 60.2 6.6
 PHE-51   ARG-52  6.9 16.9 -10.4 -7.7 124.9 84.3 -5.1
 ARG-52   LYS-53  7.9 20.4 36.5 -13.7 89.1 155.2 -6.0
 LYS-53   MET-54  6.1 20.2 11.8 -8.5 64.5 98.8 7.8
 MET-54   PHE-55  2.7 17.8 39.7 -23.7 8.2 71.3 13.4
 PRO-72   LEU-73  13.2 12.7 150.9 23.5 11.9 7.4 130.4
 LEU-73   ILE-74  11.7 11.2 21.8 -13.2 59.3 56.9 4.2
 ILE-74   THR-75  10.1 10.9 -10.5 15.9 94.6 106.2 -1.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ALA-255   THR-256  12.8 11.4 -12.2 -14.9 121.9 130.6 -14.7
 THR-256   ARG-257  15.4 14.1 31.5 -40.1 60.7 72.1 -5.2
 ARG-257   ALA-258  13.8 13.2 -169.7 -21.4 144.9 126.5 124.9
 ALA-258   GLY-259  11.2 11.5 149.3 141.4 43.5 113.7 -11.2
 GLY-259   ALA-260  14.2 9.5 90.8 4.5 52.2 172.7 -39.5
 ALA-260   LEU-261  16.0 9.6 -5.2 -30.1 120.5 65.3 -13.7
 LEU-261   ALA-262  12.9 6.6 66.1 27.6 29.2 65.6 66.1
 ALA-262   GLY-263  11.6 8.9 -63.5 10.4 136.6 71.4 -10.1
 GLY-263   ILE-264  14.8 9.9 20.8 0.4 63.9 130.6 0.0
 ILE-264   SER-265  14.7 6.8 70.8 25.1 60.5 56.7 73.3
 SER-265   PRO-266  11.3 6.3 92.7 12.3 32.1 122.4 -159.3
 PRO-266   MET-267  12.1 6.0 17.5 30.7 50.3 112.0 -15.2
 MET-267   PHE-268  9.6 9.1 -132.8 -45.2 148.0 131.2 104.8
 PHE-268   GLN-269  11.5 11.2 7.8 -22.7 28.9 25.3 -7.9

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees