Glycogen Synthase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  THR-209   ALA-210  4.4 4.7 6.1 -6.1 95.4 95.2 37.1  ALA-210   VAL-211  1.9 2.3 -1.4 -12.2 22.9 24.7 81.6  VAL-211   SER-212  1.9 2.1 11.8 0.3 99.7 101.1 -59.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-219   THR-220  5.0 5.1 1.7 -8.7 155.3 153.8 40.3  THR-220   GLU-221  3.5 3.7 18.8 -24.6 126.5 124.5 16.8  GLU-221   PRO-222  7.1 7.4 1.0 2.2 96.0 95.8 31.4  PRO-222   GLN-223  8.8 9.6 0.1 -5.7 55.1 46.1 7.0  GLN-223   PHE-224  11.9 12.2 14.1 -30.8 38.9 34.6 -85.4  PHE-224   ALA-225  9.8 10.2 18.9 -32.1 97.7 100.8 114.2  ALA-225   TYR-226  8.1 8.6 -15.5 14.4 86.9 61.7 58.6  TYR-226   GLY-227  11.6 11.9 -14.7 42.0 42.0 46.7 -117.3  GLY-227   MET-228  10.5 10.4 -22.3 13.0 92.4 98.6 5.7  MET-228   GLU-229  7.8 8.0 -26.2 4.1 75.3 78.5 5.7  GLU-229   GLY-230  5.0 5.1 0.9 -9.3 123.5 127.8 39.4  GLY-230   LEU-231  3.9 3.6 23.9 -10.9 60.4 68.6 -1.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-244   LEU-245  3.3 3.3 -19.3 8.3 53.7 53.0 94.0  LEU-245   ASN-246  2.4 2.2 -2.0 14.7 44.0 38.7 -141.4  ASN-246   GLY-247  0.6 0.8 -17.4 9.6 25.5 32.8 151.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PHE-460   SER-461  5.5 4.6 51.6 -22.4 65.9 67.4 92.1  SER-461   TRP-462  3.5 3.9 -36.9 -3.9 49.4 53.0 145.4  TRP-462   GLN-463  3.9 4.0 -7.9 -8.0 83.9 86.2 21.6  GLN-463   VAL-464  7.4 7.6 4.3 -3.6 147.1 131.7 36.4  VAL-464   ALA-465  7.3 8.0 8.0 0.9 142.6 152.9 -57.1  ALA-465   ALA-466  5.6 5.9 1.6 -15.0 92.4 101.6 32.6  ALA-466   LYS-467  8.7 8.7 30.6 -12.7 120.9 111.3 -44.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees