Pyruvate Kinase, M2 Isozyme

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 THR-113   LYS-114  6.3 5.9 13.3 -33.5 129.6 128.5 86.7
 LYS-114   GLY-115  5.8 4.9 4.7 -33.4 107.4 104.4 34.9
 GLY-115   PRO-116  2.0 1.7 34.6 3.0 90.8 109.9 -47.5
 PRO-116   GLU-117  3.3 4.1 -83.6 73.3 29.3 36.7 32.7
 GLU-117   ILE-118  6.0 5.8 -10.4 -9.6 95.3 90.7 15.9
 ILE-118   ARG-119  9.5 9.4 9.6 -16.7 108.7 107.7 17.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ASP-216   LEU-217  11.5 11.8 -6.3 -13.4 44.9 46.2 27.9
 LEU-217   PRO-218  8.7 8.6 -15.8 -15.7 55.6 72.7 72.7
 PRO-218   ALA-219  7.1 6.8 -5.5 -5.0 116.0 106.3 -14.8
 ALA-219   VAL-220  5.5 4.3 -22.1 37.0 83.7 50.2 -34.1
 VAL-220   SER-221  2.0 2.5 -31.3 -22.3 72.0 65.6 108.5
 SER-221   GLU-222  3.1 3.4 -3.8 23.8 24.4 39.0 -68.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees