Foki Restriction Endonuclease

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-191   PHE-192  6.4 7.4 45.1 -3.7 124.8 122.3 -173.7  PHE-192   THR-193  5.5 6.4 -1.5 0.2 103.4 104.3 6.0  THR-193   SER-194  2.4 3.2 -8.0 12.1 34.4 33.7 -7.3  SER-194   LEU-195  4.0 4.3 4.6 12.6 77.6 87.8 -26.7  LEU-195   PRO-196  6.0 5.3 7.4 -20.8 103.3 88.2 5.5  PRO-196   GLU-197  9.6 9.0 -8.9 -6.3 30.1 34.6 130.2  GLU-197   GLY-198  10.8 11.0 24.6 -9.0 86.5 83.0 1.5  GLY-198   ILE-199  14.3 14.1 -23.5 20.9 108.6 83.3 -57.3  ILE-199   LEU-200  13.2 13.2 -15.3 21.8 49.5 25.2 -53.5  LEU-200   LEU-201  11.3 11.4 -25.3 11.5 39.6 49.0 97.5  LEU-201   ASP-202  13.8 14.2 -16.2 15.5 103.7 102.8 19.2  ASP-202   THR-203  16.5 16.8 -17.4 8.7 83.3 70.5 90.4  THR-203   LEU-204  15.3 15.9 -18.2 30.5 14.3 3.2 -130.8  LEU-204   ALA-205  14.9 15.0 2.6 -2.2 113.5 109.0 -12.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees