Myosin Va

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-460   LEU-461  9.8 9.0 -10.5 15.5 95.2 103.6 80.6  LEU-461   GLN-462  6.9 6.1 -11.5 -1.4 163.0 160.0 -50.0  GLN-462   GLN-463  7.1 6.5 9.4 -2.3 81.2 82.0 -40.4  GLN-463   GLN-464  7.6 7.3 -0.4 5.6 73.4 82.9 58.8  GLN-464   PHE-465  4.9 4.5 -31.2 30.9 123.2 138.6 46.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TYR-477   MET-478  13.6 13.4 -0.1 -6.8 102.4 95.5 23.8  MET-478   LYS-479  16.9 16.8 6.2 -11.6 103.8 99.7 -4.0  LYS-479   GLU-480  17.8 17.3 35.5 -53.7 172.7 159.9 237.6  GLU-480   GLN-481  16.9 17.0 21.8 -3.2 120.1 125.7 -181.6  GLN-481   ILE-482  18.8 18.4 7.0 -21.6 132.7 132.5 164.3  ILE-482   PRO-483  16.2 15.6 3.9 4.9 49.1 72.3 149.9  ASP-488   PHE-489  10.6 8.7 -20.3 16.9 146.7 155.5 91.1  PHE-489   TYR-490  10.0 9.3 -2.3 -4.6 83.8 96.5 48.9  TYR-490   ASP-491  10.1 9.1 17.0 -27.6 19.0 20.8 -102.0  ASP-491   ASN-492  12.1 11.6 -20.9 -5.6 108.5 110.5 -88.9  ASN-492   GLN-493  12.8 12.6 25.3 -8.3 42.8 34.8 196.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ARG-692   ILE-693  6.5 6.5 13.1 -2.5 91.6 96.8 1.7  ILE-693   SER-694  4.8 4.9 5.5 -27.6 62.4 63.0 -177.6  SER-694   ALA-695  1.2 1.2 27.5 -3.3 8.4 4.3 265.0  ALA-695   ALA-696  2.8 2.5 -13.8 20.3 115.5 118.9 77.2  ALA-696   GLY-697  4.9 4.6 -1.3 17.6 93.0 89.4 -71.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees