Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 TYR-166   ARG-167  13.0 12.6 6.6 -1.3 64.3 64.4 12.4
 ARG-167   ASP-168  10.0 9.7 3.8 -5.6 28.4 25.5 -5.9
 ASP-168   GLN-169  9.0 8.9 -2.3 11.2 90.2 99.9 9.8
 GLN-169   PRO-170  5.8 5.4 5.3 -4.4 148.3 142.1 -4.1
 PRO-170   HIS-171  3.2 2.8 -3.2 -0.4 34.9 34.9 21.6
 HIS-171   PRO-172  4.1 3.3 -1.0 7.4 84.3 83.7 -25.4
 PRO-172   PHE-173  4.5 3.5 -10.7 -0.9 56.4 63.5 32.9
 PHE-173   ILE-174  2.0 1.3 0.8 3.3 55.1 49.9 13.1
 ILE-174   THR-175  1.5 2.1 -0.6 4.1 124.9 123.3 11.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 ASP-214   LEU-215  6.4 6.1 -1.7 -0.6 87.7 90.9 2.3
 LEU-215   VAL-216  3.4 2.8 3.6 9.3 37.7 32.5 40.1
 VAL-216   ASP-217  1.3 1.4 1.8 -4.8 35.0 42.9 -12.6
 ASP-217   LYS-218  3.9 4.0 4.2 4.6 87.4 91.4 7.5
 LYS-218   ILE-219  5.4 5.1 2.3 -7.9 79.2 68.8 7.6
 ILE-219   ILE-220  4.2 3.8 -2.1 7.5 170.1 178.8 12.5
 ILE-220   ALA-221  2.9 2.7 11.0 -9.4 60.1 65.9 6.7
 ALA-221   ASP-222  5.7 6.0 9.6 -13.5 95.9 95.9 -4.0
 ASP-222   ARG-223  8.3 8.1 6.1 4.5 55.6 48.8 23.2
 ARG-223   LYS-224  8.0 7.4 -3.9 0.4 168.7 154.5 -14.0
 LYS-224   ALA-225  6.9 6.9 6.8 -7.1 74.8 82.5 -21.7
 ALA-225   SER-226  10.2 10.4 15.7 -19.4 96.3 85.8 -21.2
 GLN-229   SER-230  15.8 14.7 0.7 -7.4 148.5 143.8 24.9
 SER-230   ASP-231  14.9 13.9 0.3 -2.5 75.9 73.3 -19.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees