Ubiquitin-Activating Enzyme E1 1

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TRP-917   ASP-918  4.8 4.7 9.8 -9.9 72.4 72.0 5.9  ASP-918   ARG-919  3.4 2.6 3.3 4.4 50.9 52.6 29.1  ARG-919   TYR-920  2.6 1.9 -4.5 11.8 109.8 108.0 18.4  TYR-920   ASN-921  5.7 5.1 18.4 -14.7 10.4 14.5 10.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees