Translation Initiation Factor If2/eif5b

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 VAL-445   ARG-446  16.5 16.3 -11.2 1.0 70.1 68.7 68.1
 ARG-446   GLY-447  15.4 14.6 4.4 3.7 175.7 171.9 -71.6
 GLY-447   ILE-448  16.7 16.4 0.5 -3.3 117.8 124.4 4.8
 ILE-448   GLU-449  15.7 15.7 -14.4 12.4 89.2 83.0 6.9
 GLU-449   GLU-450  12.1 12.0 0.5 4.0 134.7 130.6 -22.3
 GLU-450   GLU-451  12.3 12.0 6.3 -4.5 154.2 160.4 -16.1
 GLU-451   LYS-452  13.7 13.5 -21.9 13.8 76.9 70.5 17.9
 LYS-452   LYS-453  10.9 11.2 13.8 -7.2 110.3 100.9 -38.4
 LYS-453   LYS-454  7.8 8.1 18.4 -13.7 154.3 146.8 -41.6
 LYS-454   LYS-455  9.5 9.8 -19.3 4.2 43.4 36.7 82.4
 LYS-455   TRP-456  9.6 9.6 30.5 9.0 103.5 88.8 -59.6
 TRP-456   MET-457  6.2 6.2 -23.3 2.7 72.9 56.5 67.4
 MET-457   GLU-458  5.1 5.2 -8.8 11.7 6.4 9.6 -15.9
 GLU-458   ALA-459  6.5 6.6 0.8 -13.7 118.7 116.2 35.3
 ALA-459   ILE-460  3.9 4.2 12.4 -14.9 83.1 79.5 1.8
 ILE-460   ILE-461  1.3 1.0 -10.4 7.9 48.2 53.1 19.8

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Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees