DynDom - Protein Domain Motions

A Type Vi-A Crispr-Cas Rna-Guided RNA Ribonuclease, Cas13a

(All numbering and residues are taken from first PDB file)

Run Details Domains Sequence Morph Domain Pairs Contact Graph

DynDom run details

Property	Value
Name	A Type Vi-A Crispr-Cas Rna-Guided RNA Ribonuclease, Cas13a
Conformer 1 (PDB)	5xwy (A)
Conformer 2 (PDB)	5xwp (A)
Window Length	5
Minimum ratio	1.0
Minimum domain size	20

Domains

Domain	Size	Backbone RMSD (A)	Residues
1	861	2.82	3 - 511 724 - 728 732 - 732 749 - 1150
2	208	2.19	512 - 723 729 - 731 733 - 748

Sequence

`________________________________________________________________________________________________________________________` `5xwy(A) : MKVTKVGGISHKKYTSEGRLVKSESEENRTDERLSALLNMRLDMYI----------nQKRIGKLKKFFSNKMVYLKDNTLSLKNG-------------------fAVLKKIYLNEn--SE` `:` `5xwp(A) : SkVTKVGGISHKKYTSEGRLVKSESEENRTDERLSALLN-rLD-yIKNPSSTETKENQKRIGKLKKFFSNK-vYLKDNTLSLKNGKKENIDREYSETdVRDKKNFAVLKKIYLNENVNSE` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `5xwy(A) : ELEVFRNDIKKKLNKINSLKYSFEKNKANYQKINENNIEKVEGKSKRNIIYDYYRESAKRDAYVSNVKEAFDKLYKEEDIAKLVLEIENLTKLEKYKIREFYHEIIGRKNDKENFAKIIY` `:` `5xwp(A) : ELEVFRNDIKKKLNKINSLKYSFEKNKANYQKINENNIEKVEGKSKRNIIYDYYRESAKRDAYVSNVKEAFDKLYKEEDIAKLVLEIENLTKLEKYKIREFYHEIIGRKNDKENFAKIIY` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `5xwy(A) : EEIQNVNNMKELIEKVPDMSELKKSQVFYKYYLDKEELNDKNIKYAFCHFVEIEMSQLLKNYVYKRLSNISNDKIKRIFEYQNLKKLIENKLLNKLDTYVRNCGKYNYYLQDGEIATSDF` `:` `5xwp(A) : EEIQNVNN-kELIEKVPD-sELKKSQVFYKYYLDKEELNDKNIKYAFCHFVEIE-sQLLKNYVYKR----sNDKIKRIFEYQNLKKLIENKLLNKLDTYVRNCGKYNYYLQDGEIATSDF` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `5xwy(A) : IARNRQNEAFLRNIIGVSSVAYFSLRNILETENENDITGRMRGKTVKNNKGEEKYVSGEVDKIYNENKKNEVKENLKMFYSYDFNMDNKNEIEDFFANIDEAISSIRHGIVHFNLELEGK` `:` `5xwp(A) : IARNRQNEAFLRNIIGVSSVAYFSLRNILETENENDITGR-rGKTVKNNKGEEKYVSGEVDKIYNENKKNEVKENLK-fYSYDFN-dNKNEIEDFFANIDEAISSIRHGIVHFNLELEGK` `________________________________________________________________________________________________________________________` `_______________________511______________________________________________________________________________________________` `5xwy(A) : DIFAFKNIAPSEISKKMFQNEINEKKLKLKIFRQLNSANVFRYLEKYKILNYLKRTRFEFVNKNIPFVPSFTKLYSRIDDLKNSLGIYWKTPKTNDDNKTKEIIDAQIYLLKNIYYGEFL` `:` `5xwp(A) : DIFAFKNIAPSEISKK-fQNEINEKKLKLKIFRQLNSANVFRYLEKYKILNYLKRTRFEFVNKNIPFVPSFTKLYSRIDDLKNSLGIYWKTPKTNDDNKTKEIIDAQIYLLKNIYYGEFL` `_______________________511______________________________________________________________________________________________` `___________________________________________________________________________________________________________________723__` `5xwy(A) : NYFMSNNGNFFEISKEIIELNKNDKRNLKTGFYKLQKFEDIQEKIPKEYLANIQSLYMINAGNQDEEEKDTYIDFIQKIFLKGFMTYLANNGRLSLIYIGSDEETNTSLAEKKQEFDKFL` `:` `5xwp(A) : NYF-sNNGNFFEISKEIIELNKND-------------fEDIQEKIPKEYLANIQSLY-iNAG-------dTYIDFIQKIFLKGF-tYLANNGRLSLIYIGSDEETNTSLAEKKQEFDKFL` `___________________________________________________________________________________________________________________723__` `728_________________748_________________________________________________________________________________________________` `5xwy(A) : KKYEQNNNIKIPYEINEFLREIKLGNILKYTERLNMFYLILKLLNHKELTNLKGSLEKYQSANKEEAFSDQLELINLLNLDNNRVTEDFELEADEIGKFLDFNGNKVKDNKELKKFDTNK` `:` `5xwp(A) : KKYEQNNNIKIPYEINEFLREIKLGNILKYTERLN-fYLILKLLNHKELTNLKGSLEKYQSANKEEAFSDQLELINLLNLDNNRVTEDFELEADEIGKFLDFNGNKVKDNKELKKFDTNK` `728_________________748_________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `5xwy(A) : IYFDGENIIKHRAFYNIKKYGMLNLLEKIADKAGYKISIEELKKYSNKKNEIEKNHKMQENLHRKYARPRKDEKFTDEDYESYKQAIENIEEYTHLKNKVEFNELNLLQGLLLRILHRLV` `:` `5xwp(A) : IYFDGENIIKHRAFYNIKKYG-lNLLEKIADKAGYKISIEELKKYSNKKNEIEKNHK-qENLHRKYARPRKDEKFTDEDYESYKQAIENIEEYTHLKNKVEFNELNLLQGLLLRILHRLV` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `5xwy(A) : GYTSIWERDLRFRLKGEFPENQYIEEIFNFENKKNVKYKGGQIVEKYIKFYKELHQNDEVKINKYSSANIKVLKQEKKDLYIANYIAAFNYIPHAEISLLEVLENLRKLLSYDRKLKNAV` `:` `5xwp(A) : GYTSIWERDLRFRLKGEFPENQYIEEIFNFENKKNVKYKGGQIVEKYIKFYKELHQNDEVKINKYSSANIKVLKQEKKDLYIANYIAAFNYIPHAEISLLEVLENLRKLLSYDRKLKNAV` `________________________________________________________________________________________________________________________` `____________________________________________________________________` `5xwy(A) :` `MKSVVDILKEYGFVATFKIGADKKIGIQTLESEKIVHLKNLKKKKLMTDRNSEELCKLVKIMFEYKME` `:` `5xwp(A) :` `-kSVVDILKEYGFVATFKIGADKKIGIQTLESEKIVHLKNLKKKKL-tDRNSEELCKLVKI-fEYKe*` `____________________________________________________________________`

Morph

This morph was created using the MorphIt_Pro protein morphing technique.
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Domain Pairs

Property	Value
Fixed Domain ( blue )	1
Moving Domain ( red )	2
Rotation Angle (deg)	25.3
Translation (A)	-2.0
Closure (%)	99.8
Bending Residues ( green )	511 - 512 723 - 724 728 - 729 731 - 733 748 - 749
Bending Region Analysis

Dynamic Contact Graph

Conformer 1 Contact:
Residue 1 ———› Residue 2

Conformer 2 Contact:
Residue 2 ———› Residue 1

Movement classification: Hinge

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