DynDom - Protein Domain Motions

Crispr-Associated Endonuclease Cas9/csn1

(All numbering and residues are taken from first PDB file)

Run Details Domains Sequence Morph Domain Pairs Contact Graph

DynDom run details

Property	Value
Name	Crispr-Associated Endonuclease Cas9/csn1
Conformer 1 (PDB)	4cmp (A)
Conformer 2 (PDB)	4oo8 (A)
Window Length	5
Minimum ratio	1.0
Minimum domain size	20

Domains

Domain	Size	Backbone RMSD (A)	Residues
1	372	8.95	57 - 446
2	514	8.67	6 - 55 719 - 1273 1319 - 1353
3	170	3.3	447 - 712

Sequence

`___________________________________________________55___________________________________________________________________` `4cmp(A) : KYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE------------rHPIFGNI` `:` `4oo8(A) : KKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRILYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNI` `___________________________________________________55___________________________________________________________________` `________________________________________________________________________________________________________________________` `4cmp(A) : VDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLI` `:` `4oo8(A) : VDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLI` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `4cmp(A) : ALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRkAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGAS` `:` `4oo8(A) : ALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRkAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGAS` `________________________________________________________________________________________________________________________` `___________________________________________________________________________446__________________________________________` `4cmp(A) : QEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFR-----------------------------------------` `:` `4oo8(A) : QEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASA` `___________________________________________________________________________446__________________________________________` `________________________________________________________________________________________________________________________` `4cmp(A) :` `--------------pNEKVLPKHSLLYEYFTVYNELTKV------------lSGEQKKAIVDLLFKTNRKVTVKQLKED-------------------fNASLGTYHDLLKIIKDKDFLD` `:` `4oo8(A) :` `QSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIEEFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLD` `________________________________________________________________________________________________________________________` `_____________________________________________________________________________________________________712________________` `4cmp(A) : NEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDk----TILDFLK------rNFMQLIHDDSLTFKEDIQKAQVSdSLHEHIANLAGSP` `:` `4oo8(A) : NEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQV-dSLHEHIANLAGSP` `_____________________________________________________________________________________________________712________________` `________________________________________________________________________________________________________________________` `4cmp(A) : AIKKGILQTVKVVDELVKVMGRHKPENIVIEMA-----kNSRERMKRIEEGIKELhPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTR--kN` `:` `4oo8(A) : AIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTkNSRERMKRIEEGIKELGS-eNTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKN` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `4cmp(A) : RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTK-----lSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHH` `:` `4oo8(A) : RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNT--------iREVKVITLKSKLVSDFRKDFQFYKVREINNYHH` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `4cmp(A) : AHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQyFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQT---------` `:` `4oo8(A) : AHDAYLNAVVGTALIKKYPKLESEFVYGDYKVY-iGKATAK---YFFYSNIMNFFKT--------------iETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `4cmp(A) :` `-------------------------pTVAYSVLVV------------sVKELLGITIMERSSFEKNPIDFLEAKG-----kDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSK` `:` `4oo8(A) :` `PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSK` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `4cmp(A) : YVNFLYLASHYvEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLS*` `:` `4oo8(A) : YVNFLYLASHYpEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYE` `________________________________________________________________________________________________________________________` `_________` `4cmp(A) : *******` `:` `4oo8(A) : TRIDLSQLG` `_________`

Morph

Morph animation in progress.
Status: Initialising

This morph was created using the MorphIt_Pro protein morphing technique.
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Domain Pairs

Fixed Domain	Moving Domain	Rotation Angle	Translation (A)	Closure	Bending Residues
1	2	119.6	-19.6	98.4	55 - 67	Bending Region Analysis
1	3	178.9	-34.8	92.3	446 - 506	Bending Region Analysis
2	3	152.2	6.4	86.5	712 - 719	Bending Region Analysis

Dynamic Contact Graph

Conformer 1 Contact:
Residue 1 ———› Residue 2

Conformer 2 Contact:
Residue 2 ———› Residue 1

Movement classification: Hinge

Conformer 1 Contact:
Residue 1 ———› Residue 3

Conformer 2 Contact:
Residue 3 ———› Residue 1

Movement classification: Hinge

Conformer 1 Contact:
Residue 2 ———› Residue 3

Conformer 2 Contact:
Residue 3 ———› Residue 2

Movement classification:

No Contacts

aspartate aminotransferase

PyMOL Script

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