DynDom - Protein Domain Motions

Crispr-Associated Endonuclease Cas9/csn1

(All numbering and residues are taken from first PDB file)

Run Details Domains Sequence Morph Domain Pairs Contact Graph

DynDom run details

Property	Value
Name	Crispr-Associated Endonuclease Cas9/csn1
Conformer 1 (PDB)	4cmp (A)
Conformer 2 (PDB)	4zt0 (A)
Window Length	5
Minimum ratio	1.0
Minimum domain size	20

Domains

Domain	Size	Backbone RMSD (A)	Residues
1	526	5.68	6 - 56 720 - 1281 1341 - 1361
2	368	3.42	57 - 446
3	170	3.52	447 - 718
4	44	5.98	1282 - 1326

Sequence

`____________________________________________________56__________________________________________________________________` `4cmp(A) : KYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE------------rHPIFGNI` `:` `4zt0(A) : KKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNE-aKVDDSFFHRLEESFLVEEDKKHERHPIFGNI` `____________________________________________________56__________________________________________________________________` `________________________________________________________________________________________________________________________` `4cmp(A) : VDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLI` `:` `4zt0(A) : VDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH-iKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLI` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `4cmp(A) : ALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILR------kAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGY` `:` `4zt0(A) : ALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSAS-iKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGY` `________________________________________________________________________________________________________________________` `_________________________________________________________________________________446____________________________________` `4cmp(A) : IDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFR-----------------------------------` `:` `4zt0(A) : IDGGASQEEFYKFIKPILEK-dGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWtRKSEETITPWNFEEVVD` `_________________________________________________________________________________446____________________________________` `________________________________________________________________________________________________________________________` `4cmp(A) :` `------------------pNEKVLPKHSLLYEYFTVYNELTKV-----------lSGEQKKAIVDLLFKTNRKVTVKQLKED-------------------fNASLGTYHDLLKIIKDKD` `:` `4zt0(A) :` `KGASAQSFIERtNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGrKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKD` `________________________________________________________________________________________________________________________` `___________________________________________________________________________________________________________718__________` `4cmp(A) : FLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDk----TILDFLK------rNFMQLIHDDSLTFKEDIQKAQVS---dSLHEHIA` `:` `4zt0(A) : FLDNEENEDILEDIVLTLTLFEDRE-iEERLKTYAHLFDDKV-kQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNF-qLIHDDSLTFKEDIQKAQVSGQGDSLHEHIA` `___________________________________________________________________________________________________________718__________` `________________________________________________________________________________________________________________________` `4cmp(A) : NLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEM---------AkNSRERMKRIEEGIKEL-------hPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSF` `:` `4zt0(A) : NLAGSPAIKKGILQTVKVVDELVKV-gRHKPENIVIEaRENQTTQKGQKNSRER-kRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRD-yVDQELDINRLSDYDVDHIVPQSF` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `4cmp(A) : LKDDSIDNKVLTR--kNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTK-----lSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDF` `:` `4zt0(A) : LKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKK-kNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSR-nTKYDENDKLIREVKVITLKSKLVSDF` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `4cmp(A) : RKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQ--------yFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKV` `:` `4zt0(A) : RKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRK-iAKSEQEIGKATAKYFFYSNI-nFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKV` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `4cmp(A) : LSMPQVNIVKKTEVQT----------------------------------pTVAYSVLVV------------sVKELLGITIMERSSFEKNPIDFLEAKG-----kDLIIKLPKYSLFEL` `:` `4zt0(A) : LS-pQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITI-eRSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFEL` `________________________________________________________________________________________________________________________` `_______________________________________________________1281_________________________________________1326________________` `4cmp(A) : ENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYvEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEV` `:` `4zt0(A) : ENGRKRlASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDK-PIREQAENIIHLFTLTNLGAPAAFKY` `_______________________________________________________1266_________________________________________1310________________` `________________________________________` `4cmp(A) : LDA----------------TLIHQSITGLYETRIDLS**` `:` `4zt0(A) : FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLG` `________________________________________`

Morph

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Status: Initialising

This morph was created using the MorphIt_Pro protein morphing technique.
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Domain Pairs

Property	Value
Fixed Domain ( blue )	1
Moving Domain ( red )	2
Rotation Angle (deg)	5.0
Translation (A)	0.1
Closure (%)	93.2
Bending Residues ( green )	271 - 272 278 - 279 283 - 284 287 - 288 290 - 291 323 - 326 415 - 416 424 - 427 540 - 541 545 - 546
Bending Region Analysis

Dynamic Contact Graph

The dynamic contact graph is still being generated. Progress: Initialising

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