Type II restriction enzyme PvuII
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           ______________________________________41__________ 
 1ni0(B) : *HPDLNKLLELWPHIQEYQDLALKHGINDIFQDNGGKLLQVLLITGLTVL 
         :                                                    
 1h56(A) : SHPDLNKLLELWPHIQEYQDLALKHGINDIFQDNGGKLLQVLLITGLTVL 
           ______________________________________41__________ 

           __________________________________________________ 
 1ni0(B) : PGREGNDAVDNAGQEYELKSINIDLTKGFSTHHHMNPVIIAKFRQVPWIF 
         :                                                    
 1h56(A) : PGREGNDAVDNAGQEYELKSINIDLTKGFSTHHHMNPVIIAKYRQVPWIF 
           __________________________________________________ 

           __________________________________________________ 
 1ni0(B) : AIYRGIAIEAIYRLEPKDLEFYYDKWERKWYSDGHKDINNPKIPVKYVME 
         :                                                    
 1h56(A) : AIYRGIAIEAIYRLEPKDLEFYYDKWERKWYSDGHKDINNPKIPVKYVME 
           __________________________________________________ 

           ________                                           
 1ni0(B) : HGTKIYAA                                           
         :                                                    
 1h56(A) : HGTKIY**                                           
           ________                                           

Details

  Property   Value
 Conformer 1 1ni0(B)
 Conformer 2 1h56(A)
 Radius gyration for conformer 1 18.18 A
 Radius gyration for conformer 2 18.33 A
 EC Number 3.1.21.4  
Click here to see the DynDom results or the famliy
 Trigger ligands MG1
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact No
 Spanning ligands No
 Residues in enzyme contacting ligands ASP58  GLU68  GLY56  LYS70 
 Residues in extended bending regions contacting ligands Null
 Residues in extended bending regions making H-bonds or salt-bridges with ligands Null
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT