5-enolpyruvylshikimate-3-phosphate synthase
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           ________________18________________________________ 
 1rf6(C) : MKLKTNIRHLHGIIRVPGDKSISHRSIIFGSLAEGETKVYDILRGEDVLS 
         :                                                    
 1rf5(B) : MKLKTNIRHLHGIIRVPGDKSISHRSIIFGSLAEGETKVYDILRGEDVLS 
           ________________18________________________________ 

           __________________________________________________ 
 1rf6(C) : TMQVFRDLGVEIEDKDGVITVQGVGMAGLKAPQNALNMGNSGTSIRLISG 
         :                                                    
 1rf5(B) : TMQVFRDLGVEIEDKDGVITVQGVGMAGLKAPQNALNMGNSGTSIRLISG 
           __________________________________________________ 

           __________________________________________________ 
 1rf6(C) : VLAGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLK 
         :                                                    
 1rf5(B) : VLAGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLK 
           __________________________________________________ 

           __________________________________________________ 
 1rf6(C) : GTKNLRPIHYELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDML 
         :                                                    
 1rf5(B) : GTKNLRPIHYELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDML 
           __________________________________________________ 

           __________________________229_____________________ 
 1rf6(C) : QQFGGHLSVDGKKITVQGPQKLTGQKVVVPGDISSAAFWLVAGLIAPNSR 
         :                                                    
 1rf5(B) : QQFGGHLSVDGKKITVQGPQKLTGQKVVVPGDISSAAFWLVAGLIAPNSR 
           __________________________229_____________________ 

           __________________________________________________ 
 1rf6(C) : LVLQNVGINETRTGIIDVIRAMGGKLEITEIDPVAKSATLIVESSDLKGT 
         :                                                    
 1rf5(B) : LVLQNVGINETRTGIIDVIRAMGGKLEITEIDPVAKSATLIVESSDLKGT 
           __________________________________________________ 

           __________________________________________________ 
 1rf6(C) : EICGALIPRLIDELPIIALLATQAQGVTVIKDAEELKVKETDRIQVVADA 
         :                                                    
 1rf5(B) : EICGALIPRLIDELPIIALLATQAQGVTVIKDAEELKVKETDRIQVVADA 
           __________________________________________________ 

           __________________________________________________ 
 1rf6(C) : LNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAIAALLVADG 
         :                                                    
 1rf5(B) : LNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAIAALLVADG 
           __________________________________________________ 

           ___________________________                        
 1rf6(C) : EVELDRAEAINTSYPSFFDDLESLIHG                        
         :                                                    
 1rf5(B) : EVELDRAEAINTSYPSFFDDLESLIHG                        
           ___________________________                        

Details

  Property   Value
 Conformer 1 1rf6(C)
 Conformer 2 1rf5(B)
 Radius gyration for conformer 1 21.14 A
 Radius gyration for conformer 2 22.68 A
 EC Number 2.5.1.19  
Click here to see the DynDom results or the famliy
 Trigger ligands GPJ1628
 Trigger ligands in which conformation Conformation  1
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ARG120  ARG343  ARG385  ASN90  ASP312  GLN168  GLU340  GLY92  HIS384  LYS20  SER91  THR93 
 Residues in extended bending regions contacting ligands LYS20 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands LYS20 
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT