glutathione synthetase
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 1m0w(A) : *PSKDQLNELIQEVNQWAITNGLSMYPPKFEENPSNASVSPVTIYPTPIP 
         :                                                    
 1m0t(B) : PPSKDQLNELIQEVNQWAITNGLSMYPPKFEENPSNASVSPVTIYPTPIP 
           __________________________________________________ 

           __________________________________________________ 
 1m0w(A) : RKCFDEAVQIQPVFNELYARITQDMAQPDSYLHKTTEALALSDSEFTGKL 
         :                                                    
 1m0t(B) : RKCFDEAVQIQPVFNELYARITQDMAQPDSYL------------eFTGKL 
           __________________________________________________ 

           __________________________________________________ 
 1m0w(A) : WSLYLATLKSAQYKKQNFRLGIFRSDYLIDKKKGTEQIKQVEFNTVSVSF 
         :                                                    
 1m0t(B) : WSLYLATLKSAQYKKQNFRLGIFRSDYLIDKK---eQIKQVEFNTVSVSF 
           __________________________________________________ 

           __________________________________________________ 
 1m0w(A) : AGLSEKVDRLHSYLNRANKYDPKGPIYNDQNMVISDSGYLLSKALAKAVE 
         :                                                    
 1m0t(B) : AGLSEKVDRLHSYLNRANKYDPKGPIYNDQNMVISDSGYLLSKALAKAVE 
           __________________________________________________ 

           __________________________________________________ 
 1m0w(A) : SYKSQQs-----DPIVAFIVQRNERNVFDQKVLELNLLEKFGTKSVRLTF 
         :                                                    
 1m0t(B) : SYKSQQSSSTTSDPIVAFIVQRNERNVFDQKVLELNLLEKFGTKSVRLTF 
           __________________________________________________ 

           __________________________________________________ 
 1m0w(A) : DDVNDKLFIDDKTGKLFIRDTEQEIAVVYYRTGYTTTDYTSEKDWEARLF 
         :                                                    
 1m0t(B) : DDVNDKLFIDDKTGKLFIRDTEQEIAVVYYRTGYTTTDYTSEKDWEARLF 
           __________________________________________________ 

           ______________________________________________353_ 
 1m0w(A) : LEKSFAIKAPDLLTQLSGSKKIQQLLTDEGVLGKYISDAEKKSSLLKTFV 
         :                                                    
 1m0t(B) : LEKSFAIKAPDLLTQLSGSKKIQQLLTDEGVLGKYISDAEKKSSLLKTFV 
           _____________________________________________1353_ 

           __________________________________________________ 
 1m0w(A) : KIYPLDDTKLGREGKRLALSEPSKYVLKPQREGGGNNVYKENIPNFLKGI 
         :                                                    
 1m0t(B) : KIYPLDDTKLGREGKRLALSEPSKYVLKPQ-----nNVYKENIPNFLKGI 
           __________________________________________________ 

           _________416______________________________________ 
 1m0w(A) : EERHWDAYILMELIEPELNENNIILRDNKSYNEPIISELGIYGCVLFNDE 
         :                                                    
 1m0t(B) : EERHWDAYILMELIEPELNENNIILRDNKSYNEPIISELGIYGCVLFNDE 
           ________1416______________________________________ 

           _____________________________________              
 1m0w(A) : QVLSNEFSGSLLRSKFNTSNEGGVAAGFGCLDSIILY              
         :                                                    
 1m0t(B) : QVLSNEFSGSLLRSKF------------gCLDSIILY              
           _____________________________________              

Details

  Property   Value
 Conformer 1 1m0w(A)
 Conformer 2 1m0t(B)
 Radius gyration for conformer 1 22.74 A
 Radius gyration for conformer 2 23.97 A
 EC Number 6.3.2.3  
Click here to see the DynDom results or the famliy
 Trigger ligands ANP504
 Trigger ligands in which conformation Conformation  1
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ASN391  GLU146  GLU386  GLU416  GLU442  GLY387  ILE418  LEU132  LEU417  LYS324  LYS382  LYS469  MET415  TYR393  VAL145  VAL380 
 Residues in extended bending regions contacting ligands GLU416  ILE418  LEU417  MET415 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands GLU416  ILE418 
  Residues in extended bending regions with an H-bond between its main chain and ligands GLU416  ILE418 
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT