Sucrose-Phosphatase
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 1s2o(A) : MRQLLLISDLDNTWVGDQQALEHLQEYLGDRRGNFYLAYATGRSYHSARE 
         :                                                    
 1tj5(A) : MRQLLLISDLDNTWVGDQQALEHLQEYLGDRRGNFYLAYATGRSYHSARE 
           __________________________________________________ 

           _____________65___________________________________ 
 1s2o(A) : LQKQVGLMEPDYWLTAVGSEIYHPEGLDQHWADYLSEHWQRDILQAIADG 
         :                                                    
 1tj5(A) : LQKQVGLMEPDYWLTAVGSEIYHPEGLDQHWADYLSEHWQRDILQAIADG 
           _____________65___________________________________ 

           __________________________________________________ 
 1s2o(A) : FEALKPQSPLEQNPWKISYHLDPQACPTVIDQLTEMLKETGIPVQVIFSS 
         :                                                    
 1tj5(A) : FEALKPQSPLEQNPWKISYHLDPQACPTVIDQLTEMLKETGIPVQVIFSS 
           __________________________________________________ 

           _______160________________________________________ 
 1s2o(A) : GKDVDLLPQRSNKGNATQYLQQHLAMEPSQTLVCGDSGNDIGLFETSARG 
         :                                                    
 1tj5(A) : GKDVDLLPQRSNKGNATQYLQQHLAMEPSQTLVCGDSGNDIGLFETSARG 
           _______160________________________________________ 

           ____________________________________________       
 1s2o(A) : VIVRNAQPELLHWYDQWGDSRHYRAQSSHAGAILEAIAHFDFLS       
         :                                                    
 1tj5(A) : VIVRNAQPELLHWYDQWGDSRHYRAQSSHAGAILEAIAHFDFLS       
           ____________________________________________       

Details

  Property   Value
 Conformer 1 1s2o(A)
 Conformer 2 1tj5(A)
 Radius gyration for conformer 1 18.58 A
 Radius gyration for conformer 2 17.65 A
 EC Number 3.1.3.24  
Click here to see the DynDom results or the famliy
 Trigger ligands PO42
SUC1
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ALA40  ARG43  ASN12  ASN189  ASP11  ASP153  ASP155  ASP9  GLN107  GLU111  GLY42  LYS116  LYS152  LYS163  SER149  THR41  VAL67 
 Residues in extended bending regions contacting ligands LYS163  VAL67 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands LYS163 
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT