AROMATIC AMINO ACID AMINOTRANSFERASE
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           ____________________________34____________________ 
 2ay6(A) : MLGNLKPQAPDKILALMGEFRADPRQGKIDLGVGVYKDATGHTPIMRAVH 
         :                                                    
 1ay5(B) : MLGNLKPQAPDKILALMGEF------gKIDLGVGVYKDATGHTPIMRAVH 
           ____________________________34____________________ 

           __________________________________________________ 
 2ay6(A) : AAEQRMLETETTKTYAGLSGEPEFQKAMGELILGDGLKSETTATLATVGG 
         :                                                    
 1ay5(B) : AAEQRMLETETTKTYAGLSGEPEFQKAMGELILGDGLKSETTATLATVGG 
           __________________________________________________ 

           __________________________________________________ 
 2ay6(A) : TGALRQALELARMANPDLRVFVSDPTWPNHVSIMNFMGLPVQTYRYFDAE 
         :                                                    
 1ay5(B) : TGALRQALELARMANPDLRVFVSDPTWPNHVSIMNFMGLPVQTYRYFDAE 
           __________________________________________________ 

           __________________________________________________ 
 2ay6(A) : TRGVDFEGMKADLAAAKKGDMVLLHGCCHNPTGANLTLDQWAEIASILEK 
         :                                                    
 1ay5(B) : TRGVDFEGMKADLAAAKKGDMVLLHGCCHNPTGANLTLDQWAEIASILEK 
           __________________________________________________ 

           __________________________________________________ 
 2ay6(A) : TGALPLIDLAYQGFGDGLEEDAAGTRLIASRIPEVLIAASCSKNFGIYRE 
         :                                                    
 1ay5(B) : TGALPLIDLAYQGFGDGLEEDAAGTRLIASRIPEVLIAASCSKNFGIYRE 
           __________________________________________________ 

           __________________________________________________ 
 2ay6(A) : RTGCLLALCADAATRELAQGAMAFLNRQTYSFPPFHGAKIVSTVLTTPEL 
         :                                                    
 1ay5(B) : RTGCLLALCADAATRELAQGAMAFLNRQTYSFPPFHGAKIVSTVLTTPEL 
           __________________________________________________ 

           _______________333___339_______349________________ 
 2ay6(A) : RADWMAELEAVRSGMLRLREQLAGELRDLSGSDRFGFVAEHRGMFSRLGA 
         :                                                    
 1ay5(B) : RADWMAELEAVRSGMLRLREQLAGELRDLSGSDRFGFVAEHRGMFSRLGA 
           _______________333___339_______349________________ 

           _______________383______392_________________       
 2ay6(A) : TPEQVKRIKEEFGIYMVGDSRINIAGLNDNTIPILARAIIEVGV       
         :                                                    
 1ay5(B) : TPEQVKRIKEEFGIYMVGDSRINIAGLNDNTIPILARAIIEVGV       
           _______________383______392_________________       

Details

  Property   Value
 Conformer 1 2ay6(A)
 Conformer 2 1ay5(B)
 Radius gyration for conformer 1 21.47 A
 Radius gyration for conformer 2 21.26 A
 EC Number 2.6.1.57  
Click here to see the DynDom results or the famliy
 Trigger ligands MAE414
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ARG386  ASN194  GLY38  ILE17  LYS258  TRP140  VAL37 
 Residues in extended bending regions contacting ligands ARG386  GLY38  VAL37 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands ARG386  GLY38 
  Residues in extended bending regions with an H-bond between its main chain and ligands GLY38 
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT