Folylpolyglutamate synthase
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 1jbw(A) : MNYTETVAYIHSFPRLAKTGDHRRILTLLHALGNPQQQGRYIHVTGTNGK 
         :                                                    
 2gc5(A) : MNYTETVAYIHSFPRLAK--dHRRILTLLHALGNPQQQGRYIHVTGTNGK 
           __________________________________________________ 

           ___________________71_____________________________ 
 1jbw(A) : GSAANAIAHVLEASGLTVGLYTSPFIMRFNERIMIDHEPIPDAALVNAVA 
         :                                                    
 2gc5(A) : SSAANAIAHVLEASGLTVGLYTSPFIMRFNERIMIDHEPIPDAALVNAVA 
           ___________________71_____________________________ 

           __________________________________________________ 
 1jbw(A) : FVRAALERLQQQQADFNVTEFEFITALAYWYFRQRQVDVAVIEVGIGGDT 
         :                                                    
 2gc5(A) : FVRAALERLQQQQADFNVTEFEFITALGYWYFRQRQVDVAVIEVGIGGDT 
           __________________________________________________ 

           __________________________________________________ 
 1jbw(A) : DSTNVITPVVSVLTEVALDHQKLLGHTITAIAKHaGIIKRGIPVVTGNLV 
         :                                                    
 2gc5(A) : DSTNVITPVVSVLTEVALDHQKLLGHTITAIAKHaGIIKRGIPVVTGNLV 
           __________________________________________________ 

           __________________________________________________ 
 1jbw(A) : PDAAAVVAAKVATTGSQWLRFDRDFSVPKAKLHGWGQRFTYEDQDGRISD 
         :                                                    
 2gc5(A) : PDAAAVVAAKVATTGSQWLRFDRDFSVPKAKLHGWGQRFTYEDQDGRISD 
           __________________________________________________ 

           _______________________________________293________ 
 1jbw(A) : LEVPLVGDYQQRNMAIAIQTAKVYAKQTEWPLTPQNIRQGLAASHWPARL 
         :                                                    
 2gc5(A) : LEVPLVGDYQQRNMAIAIQTAKVYAKQTEWPLTPQNIRQGLAASHWPARL 
           _______________________________________293________ 

           __________________________________________________ 
 1jbw(A) : EKISDTPLIVIDGAHNPDGINGLITALKQLFSQPITVIAGILADKDYAAM 
         :                                                    
 2gc5(A) : EKISDTPLIVIDGAHNPDGINGLITALKQLFSQPITVIAGI-----yAAM 
           __________________________________________________ 

           __________________________________________________ 
 1jbw(A) : ADRLTAAFSTVYLVPVPGT-----rLKDSWQEALAASLNDVPDQPIVITG 
         :                                                    
 2gc5(A) : ADRLTAAFSTVYLVPVPGTPRALPrLKDSWQEALAASLNDVPDQPIVITG 
           __________________________________________________ 

           ______________                                     
 1jbw(A) : SLYLASAVRQTLLG                                     
         :                                                    
 2gc5(A) : SLYLASAVRQTLLG                                     
           ______________                                     

Details

  Property   Value
 Conformer 1 1jbw(A)
 Conformer 2 2gc5(A)
 Radius gyration for conformer 1 21.27 A
 Radius gyration for conformer 2 21.84 A
 EC Number 6.3.2.17  
Click here to see the DynDom results or the famliy
 Trigger ligands ACQ999
 Trigger ligands in which conformation Conformation  1
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ALA299  ALA315  ARG300  ASN264  ASN48  ASP313  ASP319  GLU143  GLU165  GLY314  GLY320  GLY49  GLY51  HIS316  LEU413  LYS50  PRO74  SER412  SER52  SER73  THR47  TRP297  TYR260  TYR414 
 Residues in extended bending regions contacting ligands ALA299  ARG300  PRO74  SER73  TRP297 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands SER73  TRP297 
  Residues in extended bending regions with an H-bond between its main chain and ligands SER73 
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT