shikimate 5-dehydrogenase
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 2d5c(A) : MLRFAVLGHPVAHSLSPAMHAFALESLGLEGSYEAWDTPLEALPGRLKEV 
         :                                                    
 1wxd(B) : MLRFAVLGHPVAHSLSPAMHAFALESLGLEGSYEAWDTPLEALPGRLKEV 
           __________________________________________________ 

           _________________________________________________1 
 2d5c(A) : RRAFRGVNLTLPLKEAALAHLDWVSPEAQRIGAVNTVLQVEGRLFGFNTD 
         :                                                    
 1wxd(B) : RRAFRGVNLTLPLKEAALAHLDWVSPEAQRIGAVNTVLQVEGRLFGFNTD 
           _________________________________________________1 

           02________________________________________________ 
 2d5c(A) : APGFLEALKAGGIPLKGPALVLGAGGAGRAVAFALREAGLEVWVWNRTPQ 
         :                                                    
 1wxd(B) : APGFLEALKAGGIPLKGPALVLGAGGAGRAVAFALREAGLEVWVWNRTPQ 
           02________________________________________________ 

           __________________________________________________ 
 2d5c(A) : RALALAEEFGLRAVPLEKAREARLLVNATRVGLEDPSASPLPAELFPEEG 
         :                                                    
 1wxd(B) : RALALAEEFGLRAVPLEKAREARLLVNATRVGLEDPSASPLPAELFPEEG 
           __________________________________________________ 

           ___________________________230____________________ 
 2d5c(A) : AAVDLVYRPLWTRFLREAKAKGLKVQTGLPMLAWQGALAFRLWTGLLPDP 
         :                                                    
 1wxd(B) : AAVDLVYRPLWTRFLREAKAKGLKVQTGLPMLAWQGALAFRLWTGLLPDP 
           ___________________________230____________________ 

           _____________                                      
 2d5c(A) : SGMEEAARRAL**                                      
         :                                                    
 1wxd(B) : SGMEEAARRALGV                                      
           _____________                                      

Details

  Property   Value
 Conformer 1 2d5c(A)
 Conformer 2 1wxd(B)
 Radius gyration for conformer 1 18.11 A
 Radius gyration for conformer 2 18.51 A
 EC Number 1.1.1.25  
Click here to see the DynDom results or the famliy
 Trigger ligands SKM301
 Trigger ligands in which conformation Conformation  1
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ASN58  ASN85  ASP100  GLN235  LEU59  LYS64  SER14  SER16  THR60  TYR207  VAL6 
 Residues in extended bending regions contacting ligands ASP100 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands ASP100 
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT