Poly(A) polymerase
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 2hhp(A) : *QKVFGITGPVSTVGATAAENKLNDSLIQELKKEGSFETEQETANRVQVL 
         :                                                    
 1fa0(A) : SQKVFGITGPVSTVGATAAENKLNDSLIQELKKEGSFETEQETANRVQVL 
           __________________________________________________ 

           __________________________________________________ 
 2hhp(A) : KILQELAQRFVYEVSKKKNMSDGMARDAGGKIFTYGSYRLGVHGPGSDID 
         :                                                    
 1fa0(A) : KILQELAQRFVYEVSKKKNMSDGMARDAGGKIFTYGSYRLGVHGPGSDID 
           __________________________________________________ 

           __________________________________________________ 
 2hhp(A) : TLVVVPKHVTREDFFTVFDSLLRERKELDEIAPVPDAFVPIIKIKFSGIS 
         :                                                    
 1fa0(A) : TLVVVPKHVTREDFFTVFDSLLRERKELDEIAPVPDAFVPIIKIKFSGIS 
           __________________________________________________ 

           __________________________________________________ 
 2hhp(A) : IDLICARLDQPQVPLSLTLSDKNLLRNLDEKDLRALNGTRVTDEILELVP 
         :                                                    
 1fa0(A) : IDLICARLDQPQVPLSLTLSDKNLLRNLDEKDLRALNGTRVTDEILELVP 
           __________________________________________________ 

           __________________________________________________ 
 2hhp(A) : KPNVFRIALRAIKLWAQRRAVYANIFGFPGGVAWAMLVARICQLYPNACS 
         :                                                    
 1fa0(A) : KPNVFRIALRAIKLWAQRRAVYANIFGFPGGVAWAMLVARICQLYPNACS 
           __________________________________________________ 

           __________________________________________________ 
 2hhp(A) : AVILNRFFIILSEWNWPQPVILKPIEDGPLQVRVWNPKIYAQDRSHRMPV 
         :                                                    
 1fa0(A) : AVILNRFFIILSEWNWPQPVILKPIEDGPLQVRVWNPKIYAQDRSHRMPV 
           __________________________________________________ 

           __________________________________________________ 
 2hhp(A) : ITPAYPSMCATHNITESTKKVILQEFVRGVQITNDIFSNKKSWANLFEKN 
         :                                                    
 1fa0(A) : ITPAYPSMCATHNITESTKKVILQEFVRGVQITNDIFSNKKSWANLFEKN 
           __________________________________________________ 

           __________________________________________________ 
 2hhp(A) : DFFFRYKFYLEITAYTRGSDEQHLKWSGLVESKVRLLVMKLEVLAGIKIA 
         :                                                    
 1fa0(A) : DFFFRYKFYLEITAYTRGSDEQHLKWSGLVESKVRLLVMKLEVLAGIKIA 
           __________________________________________________ 

           _________________422________________________460___ 
 2hhp(A) : HPFTKPFESSYCCPTEDDYEMIQDKY----GSHKTETALNALKpKAYLST 
         :                                                    
 1fa0(A) : HPFTKPFESSYCCPTEDDYEMIQDKlKLVTDENKEEESIKD-APKAYLST 
           _________________422________________________460___ 

           __________________________________________________ 
 2hhp(A) : MYIGLDFN----kEKVDIHIPCTEFVNLCRSFNEDYGDHKVFNLALRFVK 
         :                                                    
 1fa0(A) : MYIGLDFNIENKKEKVDIHIPCTEFVNLCRSFNEDYGDHKVFNLALRFVK 
           __________________________________________________ 

           _________________                                  
 2hhp(A) : GYDLPDEVFDENEKRPS                                  
         :                                                    
 1fa0(A) : GYDLPDEVFD*******                                  
           _________________                                  

Details

  Property   Value
 Conformer 1 2hhp(A)
 Conformer 2 1fa0(A)
 Radius gyration for conformer 1 24.25 A
 Radius gyration for conformer 2 24.96 A
 EC Number 2.7.7.19  
Click here to see the DynDom results or the famliy
 Trigger ligands 3AT604
POP817
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact No
 Spanning ligands No
 Residues in enzyme contacting ligands ALA237  ASP100  ASP102  GLY233  GLY88  LEU211  LEU92  LYS215  SER89  SER99  THR194  TYR224  TYR87  VAL234  VAL94 
 Residues in extended bending regions contacting ligands Null
 Residues in extended bending regions making H-bonds or salt-bridges with ligands Null
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT