GLUTAMATE DEHYDROGENASE
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           _______________17_________________________________ 
 1hwx(C) : ADREDDPNFFKMVEGFFDRGASIVEDKLVEDLKTRQTQEQKRNRVRGILR 
         :                                                    
 1nr1(C) : *****DPNFFKMVEGFFDRGASIVEDKLVEDLRTRESEEQKRNRVRGILR 
           _______________21_________________________________ 

           __________________________________________________ 
 1hwx(C) : IIKPCNHVLSLSFPIRRDDGSWEVIEGYRAQHSHQRTPCKGGIRYSTDVS 
         :                                                    
 1nr1(C) : IIKPCNHVLSLSFPIRRDDGSWEVIEGYRAQHSQHRTPCKGGIRYSTDVS 
           __________________________________________________ 

           __________________________________________________ 
 1hwx(C) : VDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKNYTDEDLEKITRRFTM 
         :                                                    
 1nr1(C) : VDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKNYTDNELEKITRRFTM 
           __________________________________________________ 

           __________________________________________________ 
 1hwx(C) : ELAKKGFIGPGVDVPAPNMSTGEREMSWIADTYASTIGHYDINAHACVTG 
         :                                                    
 1nr1(C) : ELAKKGFIGPGIDVPAPDMSTGEREMSWIADTYASTIGHYDINAHACVTG 
           __________________________________________________ 

           ____207___________________________________________ 
 1hwx(C) : KPISQGGIHGRISATGRGVFHGIENFIENASYMSILGMTPGFGDKTFAVQ 
         :                                                    
 1nr1(C) : KPISQGGIHGRISATGRGVFHGIENFINEASYMSILGMTPGFGDKTFVVQ 
           ____211___________________________________________ 

           __________________________________________________ 
 1hwx(C) : GFGNVGLHSMRYLHRFGAKCVAVGESDGSIWNPDGIDPKELEDFKLQHGT 
         :                                                    
 1nr1(C) : GFGNVGLHSMRYLHRFGAKCIAVGESDGSIWNPDGIDPKELEDFKLQHGS 
           __________________________________________________ 

           __________________________________________________ 
 1hwx(C) : ILGFPKAKIYEGSILEVDCDILIPAASEKQLTKSNAPRVKAKIIAEGANG 
         :                                                    
 1nr1(C) : ILGFPKAKPYEGSILEADCDILIPAASEKQLTKSNAPRVKAKIIAEGANG 
           __________________________________________________ 

           ___________________________380____________________ 
 1hwx(C) : PTTPQADKIFLERNIMVIPDLYLNAGGVTVSYFQILKNLNHVSYGRLTFK 
         :                                                    
 1nr1(C) : PTTPEADKIFLERNIMVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFK 
           ___________________________384____________________ 

           __________________________________________________ 
 1hwx(C) : YERDSNYHLLMSVQESLERKFGKHGGTIPIVPTAEFQDRISGASEKDIVH 
         :                                                    
 1nr1(C) : YERDSNYHLLMSVQESLERKFGKHGGTIPIVPTAEFQDRISGASEKDIVH 
           __________________________________________________ 

           453_________________________481___________________ 
 1hwx(C) : SGLAYTMERSARQIMRTAMKYNLGLDLRTAAYVNAIEKVFRVYNEAGVTF 
         :                                                    
 1nr1(C) : SGLAYTMEASARQIMRTAMKYNLGLDLRTAAYVNAIEKVFKVYNEAGVTF 
           457_________________________485___________________ 

           _                                                  
 1hwx(C) : T                                                  
         :                                                    
 1nr1(C) : T                                                  
           _                                                  

Details

  Property   Value
 Conformer 1 1hwx(C)
 Conformer 2 1nr1(C)
 Radius gyration for conformer 1 25.11 A
 Radius gyration for conformer 2 25.22 A
 EC Number 1.4.1.3  
Click here to see the DynDom results or the famliy
 Trigger ligands NAI20
 Trigger ligands in which conformation Conformation  1
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ALA325  ALA326  ALA348  ARG211  ARG94  ASN168  ASN254  ASN349  ASN374  GLN250  GLN330  GLU275  GLY251  GLY253  GLY274  GLY347  MET169  PHE252  SER170  SER276  SER327  THR215  VAL255 
 Residues in extended bending regions contacting ligands ARG211 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands ARG211 
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT