GUANYLATE KINASE
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           _____________________________34___________________ 
 2j41(A) : EKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQMREGEVDGV 
         :                                                    
 2j41(D) : *KGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQMREGEVDGV 
           _____________________________34___________________ 

           _________________________________________96__101__ 
 2j41(A) : DYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFLE 
         :                                                    
 2j41(D) : DYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFLE 
           _________________________________________96__101__ 

           __________________________________________________ 
 2j41(A) : IEVEGAKQVRKKFPDALFIFLAPPS---kEVEMMNLYDYVVVNDEVELAK 
         :                                                    
 2j41(D) : IEVEGAKQVRKKFPDALFIFLAPPSeARKEVEMMNLYDYVVVNDEVELAK 
           __________________________________________________ 

           _____________________________                      
 2j41(A) : NRIQCIVEAEHLKRERVEAK*********                      
         :                                                    
 2j41(D) : NRIQCIVEAEHLKRERVEAKYRKMILEAK                      
           _____________________________                      

Details

  Property   Value
 Conformer 1 2j41(A)
 Conformer 2 2j41(D)
 Radius gyration for conformer 1 16.27 A
 Radius gyration for conformer 2 15.91 A
 EC Number 2.7.4.8  
Click here to see the DynDom results or the famliy
 Trigger ligands 5GP1210
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ALA76  ARG43  ARG46  GLU103  GLU105  GLU74  GLY108  GLY84  ILE104  LYS19  SER39  THR85  TYR55  TYR78  TYR83 
 Residues in extended bending regions contacting ligands GLU103  GLU105  GLY108  ILE104 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands GLU103  GLU105 
  Residues in extended bending regions with an H-bond between its main chain and ligands GLU105 
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT