Nitrogenase Molybdenum-Iron Protein alpha cha
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 1l5h(A) : *********************************************NKKSQ 
         :                                                    
 1m1n(E) : MSREEVESLIQEVLEVYPEKARKDRNKHLAVNDPAVTQSKKCIISNKKSQ 
           __________________________________________________ 

           __________________________________________________ 
 1l5h(A) : PGLMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSRAGRRNYYIGT 
         :                                                    
 1m1n(E) : PGLMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSRAGRRNYYIGT 
           __________________________________________________ 

           __________________________________________________ 
 1l5h(A) : TGVNAFVTMNFTSDFQEKDIVFGGDKKLAKLIDEVETLFPLNKGISVQSE 
         :                                                    
 1m1n(E) : TGVNAFVTMNFTSDFQEKDIVFGGDKKLAKLIDEVETLFPLNKGISVQSE 
           __________________________________________________ 

           __________________________________________________ 
 1l5h(A) : CPIGLIGDDIESVSKVKGAELSKTIVPVRCEGFRGVSQSLGHHIANDAVR 
         :                                                    
 1m1n(E) : CPIGLIGDDIESVSKVKGAELSKTIVPVRCEGFRGVSQSLGHHIANDAVR 
           __________________________________________________ 

           __________________________________________________ 
 1l5h(A) : DWVLGKRDEDTTFASTPYDVAIIGDYNIGGDAWSSRILLEEMGLRCVAQW 
         :                                                    
 1m1n(E) : DWVLGKRDEDTTFASTPYDVAIIGDYNIGGDAWSSRILLEEMGLRCVAQW 
           __________________________________________________ 

           __________________________________________________ 
 1l5h(A) : SGDGSISEIELTPKVKLNLVHCYRSMNYISRHMEEKYGIPWMEYNFFGPT 
         :                                                    
 1m1n(E) : SGDGSISEIELTPKVKLNLVHCYRSMNYISRHMEEKYGIPWMEYNFFGPT 
           __________________________________________________ 

           ____________________________________________350___ 
 1l5h(A) : KTIESLRAIAAKFDESIQKKCEEVIAKYKPEWEAVVAKYRPRLEGKRVML 
         :                                                    
 1m1n(E) : KTIESLRAIAAKFDESIQKKCEEVIAKYKPEWEAVVAKYRPRLEGKRVML 
           ____________________________________________350___ 

           _357______________374_____________________________ 
 1l5h(A) : YIGGLRPRHVIGAYEDLGMEVVGTGYE----------------------- 
         :                                                    
 1m1n(E) : YIGGLRPRHVIGAYEDLGMEVVGTGYEFAHNDDYDRTMKEMGDSTLLYDD 
           _357______________374_____________________________ 

           _____411_____419____426___________________________ 
 1l5h(A) : ----eFEEFVKRIKPDLIGSGIKEKFIFQKMGIPFREMHSWDYSGPYHGF 
         :                                                    
 1m1n(E) : VTGYEFEEFVKRIKPDLIGSGIKEKFIFQKMGIPFREMHSWDYSGPYHGF 
           _____411_____419____426___________________________ 

           ___________________________                        
 1l5h(A) : DGFAIFARDMDMTLNNPCWKKLQAPWE                        
         :                                                    
 1m1n(E) : DGFAIFARDMDMTLNNPCWKKLQAPWE                        
           ___________________________                        

Details

  Property   Value
 Conformer 1 1l5h(A)
 Conformer 2 1m1n(E)
 Radius gyration for conformer 1 23.14 A
 Radius gyration for conformer 2 22.76 A
 EC Number 1.18.6.1  
Click here to see the DynDom results or the famliy
 Trigger ligands HCA8494
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ALA65  ARG96  GLN191  GLY424  HIS442  ILE425  LYS426 
 Residues in extended bending regions contacting ligands GLY424  ILE425  LYS426 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands ILE425 
  Residues in extended bending regions with an H-bond between its main chain and ligands ILE425 
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT